This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rosner, B. M. Articles by Spormann, A. M. Search for Related Content PubMed PubMed Citation Articles by Rosner, B. M. Articles by Spormann, A. M. Agricola Articles by Rosner, B. M. Articles by Spormann, A. M.

Next Article 

Appl. Environ. Microbiol., Nov 1997, 4139-4144, Vol 63, No. 11
Copyright © 1997, American Society for Microbiology

In Vitro Studies on Reductive Vinyl Chloride Dehalogenation by an Anaerobic Mixed Culture

BM Rosner, PL Mccarty and AM Spormann
Environmental Engineering and Science, Department of Civil and Environmental Engineering, Stanford University, Stanford, California 94305-4020

Reductive dehalogenation of vinyl chloride (VC) was studied in an anaerobic mixed bacterial culture. In growth experiments, ethene formation from VC increased exponentially at a rate of about 0.019 h(sup-1). Reductive VC dehalogenation was measured in vitro by using cell extracts of the mixed culture. The apparent K(infm) for VC was determined to be about 76 (mu)M; the V(infmax) was about 28 nmol (middot) min(sup-1) (middot) mg of protein(sup-1). The VC-dehalogenating activity was membrane associated. Propyl iodide had an inhibitory effect on the VC-dehalogenating activity in the in vitro assay. However, this inhibition could not be reversed by illumination. Cell extracts also catalyzed the reductive dehalogenation of cis-1,2-dichloroethene (cis-DCE) and, at a lower rate, of trichloroethene (TCE). Tetrachloroethene (PCE) was not transformed. The results indicate that the reductive dehalogenation of VC and cis-DCE described here is different from previously reported reductive dehalogenation of PCE and TCE.

This article has been cited by other articles:

• Muller, J. A., Rosner, B. M., von Abendroth, G., Meshulam-Simon, G., McCarty, P. L., Spormann, A. M. (2004). Molecular Identification of the Catabolic Vinyl Chloride Reductase from Dehalococcoides sp. Strain VS and Its Environmental Distribution. Appl. Environ. Microbiol. 70: 4880-4888 [Abstract] [Full Text]  
• Cupples, A. M., Spormann, A. M., McCarty, P. L. (2003). Growth of a Dehalococcoides-Like Microorganism on Vinyl Chloride and cis-Dichloroethene as Electron Acceptors as Determined by Competitive PCR. Appl. Environ. Microbiol. 69: 953-959 [Abstract] [Full Text]  
• He, J., Ritalahti, K. M., Aiello, M. R., Loffler, F. E. (2003). Complete Detoxification of Vinyl Chloride by an Anaerobic Enrichment Culture and Identification of the Reductively Dechlorinating Population as a Dehalococcoides Species. Appl. Environ. Microbiol. 69: 996-1003 [Abstract] [Full Text]  
• Hendrickson, E. R., Payne, J. A., Young, R. M., Starr, M. G., Perry, M. P., Fahnestock, S., Ellis, D. E., Ebersole, R. C. (2002). Molecular Analysis of Dehalococcoides 16S Ribosomal DNA from Chloroethene-Contaminated Sites throughout North America and Europe. Appl. Environ. Microbiol. 68: 485-495 [Abstract] [Full Text]  
• Maymó-Gatell, X., Anguish, T., Zinder, S. H. (1999). Reductive Dechlorination of Chlorinated Ethenes and 1,2-Dichloroethane by "Dehalococcoides ethenogenes" 195. Appl. Environ. Microbiol. 65: 3108-3113 [Abstract] [Full Text]