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Appl. Environ. Microbiol., Nov 1997, 4593-4596, Vol 63, No. 11
Copyright © 1997, American Society for Microbiology

Application of the extracellular alpha-amylase gene from Streptococcus bovis 148 to construction of a secretion vector for yogurt starter strains

E Satoh, Y Ito, Y Sasaki and T Sasaki
Central Research Institute, Meiji Milk Products Co., Ltd., Odawara, Japan.

Streptococcus thermophilus ATCC 19258, Lactobacillus delbrueckii subsp. bulgaricus T-11, and Lactococcus lactis subsp. lactis IL1403 were transformed with the alpha-amylase gene (amyA) from Streptococcus bovis 148 by using a wide host-range vector, and all the transformants secreted the alpha-amylase successfully. Since the promoter and the secretion signal of the amyA gene were functional in these strains, we constructed a secretion vector using the expression elements of amyA. Trials to secrete foreign enzymes in yogurt starter strains were performed using this novel secretion vector.


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