This Article Full Text (PDF) An erratum has been published Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by He, Z. Articles by Spain, J. C. Search for Related Content PubMed PubMed Citation Articles by He, Z. Articles by Spain, J. C. Agricola Articles by He, Z. Articles by Spain, J. C.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Dec 1997, 4839-4843, Vol 63, No. 12
Copyright © 1997, American Society for Microbiology

Studies of the catabolic pathway of degradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45: removal of the amino group from 2- aminomuconic semialdehyde

Z He and JC Spain
Air Force Research Laboratory, Tyndall Air Force Base, Floride 32403, USA. jspain@ccmail.aleq.tyndall.af.mil

Pseudomonas pseudoalcaligenes JS45 utilizes nitrobenzene as the sole source of nitrogen, carbon, and energy. Previous studies have shown that degradation of nitrobenzene involves the reduction of nitrobenzene to nitrosobenzene and hydroxylaminobenzene, followed by rearrangement to 2-aminophenol, which then undergoes meta ring cleavage to 2- aminomuconic semialdehyde. In the present paper, we report the enzymatic reactions responsible for the release of ammonia after ring cleavage. 2-Aminomuconic semialdehyde was oxidized to 2-aminomuconate in the presence of NAD by enzymes in crude extracts. 2-Aminomuconate was subsequently deaminated stoichiometrically to 4-oxalocrotonic acid. No cofactors are required for the deamination. Two enzymes, 2- aminomuconic semialdehyde dehydrogenase and a novel 2-aminomuconate deaminase, distinguished by partial purification of the crude extracts, catalyzed the two reactions. 4-Oxalocrotonic acid was further degraded to pyruvate and acetaldehyde. The key enzyme, 2-aminomuconate deaminase, catalyzed the hydrolytic deamination that released ammonia, which served as the nitrogen source for growth of the organism.

This article has been cited by other articles:

• Liu, L., Wu, J.-F., Ma, Y.-F., Wang, S.-Y., Zhao, G.-P., Liu, S.-J. (2007). A Novel Deaminase Involved in Chloronitrobenzene and Nitrobenzene Degradation with Comamonas sp. Strain CNB-1. J. Bacteriol. 189: 2677-2682 [Abstract] [Full Text]  
• Wu, J.-f., Jiang, C.-y., Wang, B.-j., Ma, Y.-f., Liu, Z.-p., Liu, S.-j. (2006). Novel Partial Reductive Pathway for 4-Chloronitrobenzene and Nitrobenzene Degradation in Comamonas sp. Strain CNB-1.. Appl. Environ. Microbiol. 72: 1759-1765 [Abstract] [Full Text]  
• Colabroy, K. L., Begley, T. P. (2005). Tryptophan Catabolism: Identification and Characterization of a New Degradative Pathway. J. Bacteriol. 187: 7866-7869 [Abstract] [Full Text]  
• Park, H.-S., Kim, H.-S. (2001). Genetic and Structural Organization of the Aminophenol Catabolic Operon and Its Implication for Evolutionary Process. J. Bacteriol. 183: 5074-5081 [Abstract] [Full Text]  
• Sáez, L. P., García, P., Martínez-Luque, M., Klipp, W., Blasco, R., Castillo, F. (2001). Role for draTG and rnf Genes in Reduction of 2,4-Dinitrophenol by Rhodobacter capsulatus. J. Bacteriol. 183: 1780-1783 [Abstract] [Full Text]  
• Davis, J. K., Paoli, G. C., He, Z., Nadeau, L. J., Somerville, C. C., Spain, J. C. (2000). Sequence Analysis and Initial Characterization of Two Isozymes of Hydroxylaminobenzene Mutase from Pseudomonas pseudoalcaligenes JS45. Appl. Environ. Microbiol. 66: 2965-2971 [Abstract] [Full Text]  
• He, Z., Spain, J. C. (2000). Reactions Involved in the Lower Pathway for Degradation of 4-Nitrotoluene by Mycobacterium Strain HL 4-NT-1. Appl. Environ. Microbiol. 66: 3010-3015 [Abstract] [Full Text]  
• Park, H.-S., Kim, H.-S. (2000). Identification and Characterization of the Nitrobenzene Catabolic Plasmids pNB1 and pNB2 in Pseudomonas putida HS12. J. Bacteriol. 182: 573-580 [Abstract] [Full Text]  
• Schenzle, A., Lenke, H., Spain, J. C., Knackmuss, H.-J. (1999). Chemoselective Nitro Group Reduction and Reductive Dechlorination Initiate Degradation of 2-Chloro-5-Nitrophenol by Ralstonia eutropha JMP134. Appl. Environ. Microbiol. 65: 2317-2323 [Abstract] [Full Text]  
• He, Z., Davis, J. K., Spain, J. C. (1998). Purification, Characterization, and Sequence Analysis of 2-Aminomuconic 6-Semialdehyde Dehydrogenase from Pseudomonas pseudoalcaligenes JS45. J. Bacteriol. 180: 4591-4595 [Abstract] [Full Text]  
• He, Z., Spain, J. C. (1998). A Novel 2-Aminomuconate Deaminase in the Nitrobenzene Degradation Pathway of Pseudomonas pseudoalcaligenes JS45. J. Bacteriol. 180: 2502-2506 [Abstract] [Full Text]