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Appl. Environ. Microbiol., Feb 1997, 676-678, Vol 63, No. 2
Copyright © 1997, American Society for Microbiology

Intersporal Genetic Variation of Gigaspora margarita, a Vesicular Arbuscular Mycorrhizal Fungus, Revealed by M13 Minisatellite-Primed PCR

A Zeze, E Sulistyowati, K Ophel-Keller, S Barker and S Smith
Cooperative Research Centre for Soil and Land Management, Department of Plant Science, and Department of Soil Science, University of Adelaide, Glen Osmond 5064, Adelaide, South Australia, Australia

Spores of vesicular arbuscular mycorrhizal (VAM) fungi contain thousands of nuclei. In order to understand the karyotic structure of a VAM fungus spore, the genetic variation of the first generation of spores from a VAM fungus (Gigaspora margarita) was examined. Spores originating from both single- and multispore inoculations of the species G. margarita were analyzed by M13 minisatellite-primed PCR. In both cases, different fingerprints were obtained from individual spores with few spores exhibiting similar fingerprints. These results can be explained only by a heterokaryotic status of the nuclear population within a spore.

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