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Appl. Environ. Microbiol., Mar 1997, 862-866, Vol 63, No. 3
Copyright © 1997, American Society for Microbiology

Dechlorination of Atrazine by a Rhizobium sp. Isolate

C Bouquard, J Ouazzani, J Prome, Y Michel-Briand and P Plesiat
Laboratoire de Bacteriologie, Faculte de Medecine, 25030 Besancon, Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique (CNRS), 91198 Gif sur Yvette, and Laboratoire de Pharmacologie et Toxicologie Fondamentales, CNRS, 31077 Toulouse, France

A Rhizobium sp. strain, named PATR, was isolated from an agricultural soil and found to actively degrade the herbicide atrazine. Incubation of PATR in a basal liquid medium containing 30 mg of atrazine liter(sup-1) resulted in the rapid consumption of the herbicide and the accumulation of hydroxyatrazine as the only metabolite detected after 8 days of culture. Experiments performed with ring-labeled [(sup14)C]atrazine indicated no mineralization. The enzyme responsible for the hydroxylation of atrazine was partially purified and found to consist of four 50-kDa subunits. Its synthesis in PATR was constitutive. This new atrazine hydrolase demonstrated 92% sequence identity through a 24-amino-acid fragment with atrazine chlorohydrolase AtzA produced by Pseudomonas sp. strain ADP.

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