This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Le Marrec, C.
Right arrow Articles by Fayard, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Le Marrec, C.
Right arrow Articles by Fayard, B.
Agricola
Right arrow Articles by Le Marrec, C.
Right arrow Articles by Fayard, B.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Aug 1997, 3246-3253, Vol 63, No. 8
Copyright © 1997, American Society for Microbiology

Two groups of bacteriophages infecting Streptococcus thermophilus can be distinguished on the basis of mode of packaging and genetic determinants for major structural proteins

C Le Marrec, D van Sinderen, L Walsh, E Stanley, E Vlegels, S Moineau, P Heinze, G Fitzgerald and B Fayard
Microbiology Department, University College Cork, Ireland.

A comparative study of 30 phages of Streptococcus thermophilus was performed based on DNA restriction profiles, DNA homology, structural proteins, packaging mechanisms, and host range data. All phages exhibited distinct DNA restriction profiles, with some phages displaying similarly sized restriction fragments. DNA homology was shown to be present among all 30 phages. The phages could be divided into two groups on the basis of their packaging mechanism as was derived from the appearance of submolar DNA fragments in restriction enzyme digests and the presence (cos-containing phages) or absence (pac- containing phages) of cohesive genomic extremities. Interestingly, the 19 identified cos-containing phages possessed two major structural proteins (32 and 26 kDa) in contrast to the remaining 11 pac-containing phages, which possessed three major structural proteins (41, 25, and 13 kDa). Southern hybridization demonstrated that all pac-containing phages tested contain homologs of the genes encoding the three major structural proteins of the pac-containing phage O1205, whereas all cos- containing phages tested exhibit homology to the gene specifying one of the structural components of the cos-containing phage phi 7201. Fifty- seven percent of the phages (both cos and pac containing) possessed the previously identified 2.2-kb EcoRI fragment of the temperate S. thermophilus phage Sfi18 (H. Brussow, A. Probst, M. Fremont, and J. Sidoti, Virology 200:854-857, 1994). No obvious correlation was detected between grouping based on packaging mechanism and host range data obtained with 39 industrial S. thermophilus strains.


This article has been cited by other articles:

  • del Rio, B., Martin, M. C., Martinez, N., Magadan, A. H., Alvarez, M. A. (2008). Multiplex Fast Real-Time PCR for Quantitative Detection and Identification of cos- and pac-Type Streptococcus thermophilus Bacteriophages. Appl. Environ. Microbiol. 74: 4779-4781 [Abstract] [Full Text]  
  • Deveau, H., Barrangou, R., Garneau, J. E., Labonte, J., Fremaux, C., Boyaval, P., Romero, D. A., Horvath, P., Moineau, S. (2008). Phage Response to CRISPR-Encoded Resistance in Streptococcus thermophilus. J. Bacteriol. 190: 1390-1400 [Abstract] [Full Text]  
  • Fortier, L.-C., Moineau, S. (2007). Morphological and Genetic Diversity of Temperate Phages in Clostridium difficile. Appl. Environ. Microbiol. 73: 7358-7366 [Abstract] [Full Text]  
  • Quiberoni, A., Tremblay, D., Ackermann, H.-W., Moineau, S., Reinheimer, J. A. (2006). Diversity of Streptococcus thermophilus Phages in a Large-Production Cheese Factory in Argentina.. J DAIRY SCI 89: 3791-3799 [Abstract] [Full Text]  
  • Binetti, A. G., Del Rio, B., Martin, M. C., Alvarez, M. A. (2005). Detection and Characterization of Streptococcus thermophilus Bacteriophages by Use of the Antireceptor Gene Sequence. Appl. Environ. Microbiol. 71: 6096-6103 [Abstract] [Full Text]  
  • Bolotin, A., Quinquis, B., Sorokin, A., Ehrlich, S. D. (2005). Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin. Microbiology 151: 2551-2561 [Abstract] [Full Text]  
  • Levesque, C., Duplessis, M., Labonte, J., Labrie, S., Fremaux, C., Tremblay, D., Moineau, S. (2005). Genomic Organization and Molecular Analysis of Virulent Bacteriophage 2972 Infecting an Exopolysaccharide-Producing Streptococcus thermophilus Strain. Appl. Environ. Microbiol. 71: 4057-4068 [Abstract] [Full Text]  
  • Lamothe, G., Levesque, C., Bissonnette, F., Cochu, A., Vadeboncoeur, C., Frenette, M., Duplessis, M., Tremblay, D., Moineau, S. (2005). Characterization of the cro-ori Region of the Streptococcus thermophilus Virulent Bacteriophage DT1. Appl. Environ. Microbiol. 71: 1237-1246 [Abstract] [Full Text]  
  • Casjens, S. R., Gilcrease, E. B., Winn-Stapley, D. A., Schicklmaier, P., Schmieger, H., Pedulla, M. L., Ford, M. E., Houtz, J. M., Hatfull, G. F., Hendrix, R. W. (2005). The Generalized Transducing Salmonella Bacteriophage ES18: Complete Genome Sequence and DNA Packaging Strategy. J. Bacteriol. 187: 1091-1104 [Abstract] [Full Text]  
  • Sumby, P., Waldor, M. K. (2003). Transcription of the Toxin Genes Present within the Staphylococcal Phage {phi}Sa3ms Is Intimately Linked with the Phage's Life Cycle. J. Bacteriol. 185: 6841-6851 [Abstract] [Full Text]  
  • Canchaya, C., Proux, C., Fournous, G., Bruttin, A., Brussow, H. (2003). Prophage Genomics. Microbiol. Mol. Biol. Rev. 67: 238-276 [Abstract] [Full Text]  
  • Broadbent, J. R., McMahon, D. J., Welker, D. L., Oberg, C. J., Moineau, S. (2003). Biochemistry, Genetics, and Applications of Exopolysaccharide Production in Streptococcus thermophilus: A Review. J DAIRY SCI 86: 407-423 [Abstract] [Full Text]  
  • Proux, C., van Sinderen, D., Suarez, J., Garcia, P., Ladero, V., Fitzgerald, G. F., Desiere, F., Brussow, H. (2002). The Dilemma of Phage Taxonomy Illustrated by Comparative Genomics of Sfi21-Like Siphoviridae in Lactic Acid Bacteria. J. Bacteriol. 184: 6026-6036 [Abstract] [Full Text]  
  • Rohwer, F., Edwards, R. (2002). The Phage Proteomic Tree: a Genome-Based Taxonomy for Phage. J. Bacteriol. 184: 4529-4535 [Abstract] [Full Text]  
  • Sturino, J. M., Klaenhammer, T. R. (2002). Expression of Antisense RNA Targeted against Streptococcus thermophilus Bacteriophages. Appl. Environ. Microbiol. 68: 588-596 [Abstract] [Full Text]  
  • Mahanivong, C., Boyce, J. D., Davidson, B. E., Hillier, A. J. (2001). Sequence Analysis and Molecular Characterization of the Lactococcus lactis Temperate Bacteriophage BK5-T. Appl. Environ. Microbiol. 67: 3564-3576 [Abstract] [Full Text]  
  • Lucchini, S., Desiere, F., Brussow, H. (1999). Comparative Genomics of Streptococcus thermophilus Phage Species Supports a Modular Evolution Theory. J. Virol. 73: 8647-8656 [Abstract] [Full Text]  
  • Sheehan, M. M., Stanley, E., Fitzgerald, G. F., van Sinderen, D. (1999). Identification and Characterization of a Lysis Module Present in a Large Proportion of Bacteriophages Infecting Streptococcus thermophilus. Appl. Environ. Microbiol. 65: 569-577 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»