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Appl. Environ. Microbiol., Aug 1997, 3304-3307, Vol 63, No. 8
Copyright © 1997, American Society for Microbiology

Modified Quantitative Association of Official Analytical Chemists Sporicidal Test for Liquid Chemical Germicides

N Miner, M Armstrong, CD Carr, B Maida and L Schlotfeld
MicroChem Laboratory, Fort Worth, Texas 76118

The Association of Official Analytical Chemists (AOAC) test for sporicidal activity of disinfectants (966.04) is used in the United States as the legal criteria for classifying a liquid as a chemical sterilant and also as the indicator of the highest level of disinfectant. This qualitative test contains procedures that may cause inaccurate results. A modified, quantitative version of the AOAC sporicidal test has been developed that uses a specified minimum number of spores (2.0 x 10(sup5)) of Bacillus subtilis or Clostridium sporogenes dried onto porcelain penicylinders. This modified test was validated with three commercial sterilant chemicals tested on three separate groups of spore-labeled cylinders.