This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Verheul, A.
Right arrow Articles by Abee, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Verheul, A.
Right arrow Articles by Abee, T.
Agricola
Right arrow Articles by Verheul, A.
Right arrow Articles by Abee, T.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 09 1997, 3451-3457, Vol 63, No. 9
Copyright © 1997, American Society for Microbiology

Modifications of membrane phospholipid composition in nisin-resistant Listeria monocytogenes Scott A

A Verheul, NJ Russell, R Van'T Hof, FM Rombouts and T Abee
Department of Food Science, Agricultural University Wageningen, The Netherlands.

A nisin-resistant (NISr) variant of Listeria monocytogenes Scott A was isolated by stepwise exposure to increasing concentrations of nisin in brain heart infusion (BHI) broth. The NISr strain was about 12 times more resistant to nisin than was the wild-type (WT) strain. Accordingly, higher nisin concentrations were required to dissipate both components of the proton motive force in the NISr strain than in the WT strain. Comparison of the membrane fatty acyl composition of the sensitive strain with that of its NISr derivative revealed no significant differences. From phospholipid head group composition analysis and phospholipid biosynthesis measurements during growth in the absence and presence of nisin, it could be inferred that the NISr strain produces relatively more phosphatidylglycerol (PG) and less diphosphatidylglycerol (DPG) than the parent strain does. Monolayer studies with pure lipid extracts from both strains showed that nisin interacted more efficiently with lipids derived from the WT strain than with those derived from the NISr strain, reflecting qualitative differences in nisin sensitivity. Involvement of the cell wall in acquisition of nisin resistance was excluded, since the WT and NISr strains showed a comparable sensitivity to lysozyme. Recently, it has been demonstrated that nisin penetrates more deeply into lipid monolayers of DPG than those of other lipids including PG, phosphatidylcholine, phosphatidylethanolamine, monogalactosyldiacylglycerol, and digalactosyldiacylglycerol (R.A. Demel, T. Peelen, R.J. Siezen, B. de Kruijff, and O.P. Kuipers, Eur. J.Biochem. 235:267-274, 1996). Collectively, the mechanism of nisin resistance in this L. monocytogenes NISr strain is attributed to a reduction in the DPG content of the cytoplasmic membrane.


This article has been cited by other articles:

  • Mastronicolis, S. K., Arvanitis, N., Karaliota, A., Magiatis, P., Heropoulos, G., Litos, C., Moustaka, H., Tsakirakis, A., Paramera, E., Papastavrou, P. (2008). Coordinated Regulation of Cold-Induced Changes in Fatty Acids with Cardiolipin and Phosphatidylglycerol Composition among Phospholipid Species for the Food Pathogen Listeria monocytogenes. Appl. Environ. Microbiol. 74: 4543-4549 [Abstract] [Full Text]  
  • Guinane, C. M., Cotter, P. D., Lawton, E. M., Hill, C., Ross, R. P. (2007). Insertional Mutagenesis To Generate Lantibiotic Resistance in Lactococcus lactis. Appl. Environ. Microbiol. 73: 4677-4680 [Abstract] [Full Text]  
  • Bierne, H., Cossart, P. (2007). Listeria monocytogenes Surface Proteins: from Genome Predictions to Function. Microbiol. Mol. Biol. Rev. 71: 377-397 [Abstract] [Full Text]  
  • Jydegaard-Axelsen, A.-M., Hoiby, P. E., Holmstrom, K., Russell, N., Knochel, S. (2004). CO2- and Anaerobiosis-Induced Changes in Physiology and Gene Expression of Different Listeria monocytogenes Strains. Appl. Environ. Microbiol. 70: 4111-4117 [Abstract] [Full Text]  
  • Gravesen, A., Kallipolitis, B., Holmstrom, K., Hoiby, P. E., Ramnath, M., Knochel, S. (2004). pbp2229-Mediated Nisin Resistance Mechanism in Listeria monocytogenes Confers Cross-Protection to Class IIa Bacteriocins and Affects Virulence Gene Expression. Appl. Environ. Microbiol. 70: 1669-1679 [Abstract] [Full Text]  
  • Gaussier, H., Lefevre, T., Subirade, M. (2003). Binding of Pediocin PA-1 with Anionic Lipid Induces Model Membrane Destabilization. Appl. Environ. Microbiol. 69: 6777-6784 [Abstract] [Full Text]  
  • Sakayori, Y., Muramatsu, M., Hanada, S., Kamagata, Y., Kawamoto, S., Shima, J. (2003). Characterization of Enterococcus faecium mutants resistant to mundticin KS, a class IIa bacteriocin. Microbiology 149: 2901-2908 [Abstract] [Full Text]  
  • Katla, T., Naterstad, K., Vancanneyt, M., Swings, J., Axelsson, L. (2003). Differences in Susceptibility of Listeria monocytogenes Strains to Sakacin P, Sakacin A, Pediocin PA-1, and Nisin. Appl. Environ. Microbiol. 69: 4431-4437 [Abstract] [Full Text]  
  • Vadyvaloo, V., Hastings, J. W., van der Merwe, M. J., Rautenbach, M. (2002). Membranes of Class IIa Bacteriocin-Resistant Listeria monocytogenes Cells Contain Increased Levels of Desaturated and Short-Acyl-Chain Phosphatidylglycerols. Appl. Environ. Microbiol. 68: 5223-5230 [Abstract] [Full Text]  
  • Benech, R.-O., Kheadr, E. E., Lacroix, C., Fliss, I. (2002). Antibacterial Activities of Nisin Z Encapsulated in Liposomes or Produced In Situ by Mixed Culture during Cheddar Cheese Ripening. Appl. Environ. Microbiol. 68: 5607-5619 [Abstract] [Full Text]  
  • Benech, R.-O., Kheadr, E. E., Laridi, R., Lacroix, C., Fliss, I. (2002). Inhibition of Listeria innocua in Cheddar Cheese by Addition of Nisin Z in Liposomes or by In Situ Production in Mixed Culture. Appl. Environ. Microbiol. 68: 3683-3690 [Abstract] [Full Text]  
  • Karatzas, K. A. G., Bennik, M. H. J. (2002). Characterization of a Listeria monocytogenes Scott A Isolate with High Tolerance towards High Hydrostatic Pressure. Appl. Environ. Microbiol. 68: 3183-3189 [Abstract] [Full Text]  
  • Nichols, D. S., Presser, K. A., Olley, J., Ross, T., McMeekin, T. A. (2002). Variation of Branched-Chain Fatty Acids Marks the Normal Physiological Range for Growth in Listeria monocytogenes. Appl. Environ. Microbiol. 68: 2809-2813 [Abstract] [Full Text]  
  • Limonet, M., Revol-Junelles, A.-M., Milliere, J.-B. (2002). Variations in the Membrane Fatty Acid Composition of Resistant or Susceptible Leuconostoc or Weissella Strains in the Presence or Absence of Mesenterocin 52A and Mesenterocin 52B Produced by Leuconostoc mesenteroides subsp. mesenteroides FR52. Appl. Environ. Microbiol. 68: 2910-2916 [Abstract] [Full Text]  
  • Gravesen, A., Jydegaard Axelsen, A.-M., Mendes da Silva, J., Hansen, T. B., Knochel, S. (2002). Frequency of Bacteriocin Resistance Development and Associated Fitness Costs in Listeria monocytogenes. Appl. Environ. Microbiol. 68: 756-764 [Abstract] [Full Text]  
  • Ryan, M. P., Ross, R. P., Hill, C. (2001). Strategy for Manipulation of Cheese Flora Using Combinations of Lacticin 3147-Producing and -Resistant Cultures. Appl. Environ. Microbiol. 67: 2699-2704 [Abstract] [Full Text]  
  • Pol, I. E., van Arendonk, W. G. C., Mastwijk, H. C., Krommer, J., Smid, E. J., Moezelaar, R. (2001). Sensitivities of Germinating Spores and Carvacrol-Adapted Vegetative Cells and Spores of Bacillus cereus to Nisin and Pulsed-Electric-Field Treatment. Appl. Environ. Microbiol. 67: 1693-1699 [Abstract] [Full Text]  
  • Budde, B. B., Jakobsen, M. (2000). Real-Time Measurements of the Interaction between Single Cells of Listeria monocytogenes and Nisin on a Solid Surface. Appl. Environ. Microbiol. 66: 3586-3591 [Abstract] [Full Text]  
  • Ramnath, M., Beukes, M., Tamura, K., Hastings, J. W. (2000). Absence of a Putative Mannose-Specific Phosphotransferase System Enzyme IIAB Component in a Leucocin A-Resistant Strain of Listeria monocytogenes, as Shown by Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis. Appl. Environ. Microbiol. 66: 3098-3101 [Abstract] [Full Text]  
  • Gravesen, A., Warthoe, P., Knøchel, S., Thirstrup, K. (2000). Restriction fragment differential display of pediocin-resistant Listeria monocytogenes 412 mutants shows consistent overexpression of a putative {beta}-glucoside-specific PTS system. Microbiology 146: 1381-1389 [Abstract] [Full Text]  
  • Mazzotta, A. S., Montville, T. J. (1999). Characterization of Fatty Acid Composition, Spore Germination, and Thermal Resistance in a Nisin-Resistant Mutant of Clostridium botulinum 169B and in the Wild-Type Strain. Appl. Environ. Microbiol. 65: 659-664 [Abstract] [Full Text]  
  • Eichenbaum, Z., Federle, M. J., Marra, D., de Vos, W. M., Kuipers, O. P., Kleerebezem, M., Scott, J. R. (1998). Use of the Lactococcal nisA Promoter To Regulate Gene Expression in Gram-Positive Bacteria: Comparison of Induction Level and Promoter Strength. Appl. Environ. Microbiol. 64: 2763-2769 [Abstract] [Full Text]  
  • Wiedemann, I., Breukink, E., van Kraaij, C., Kuipers, O. P., Bierbaum, G., de Kruijff, B., Sahl, H.-G. (2001). Specific Binding of Nisin to the Peptidoglycan Precursor Lipid II Combines Pore Formation and Inhibition of Cell Wall Biosynthesis for Potent Antibiotic Activity. J. Biol. Chem. 276: 1772-1779 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»