This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grant, M. A. Search for Related Content PubMed PubMed Citation Articles by Grant, M. A. Agricola Articles by Grant, M. A.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 09 1997, 3526-3530, Vol 63, No. 9
Copyright © 1997, American Society for Microbiology

A new membrane filtration medium for simultaneous detection and enumeration of Escherichia coli and total coliforms

MA Grant
Hach Company, Ames, Iowa 50010, USA.

Recovery of total coliforms and Escherichia coli on a new membrane filtration (MF) medium was evaluated with 25 water samples from seven states. Testing of the new medium, m-ColiBlue24 broth, was conducted according to a U.S. Environmental Protection Agency protocol. For comparison, this same protocol was used to measure recovery of total coliforms and E. coli with two standard MF media, m-Endo broth and mTEC broth. E. coli recovery on the new medium was also compared to recovery on nutrient agar supplemented with 4-methylumbelliferyl-beta-D- glucuronide. Comparison of specificity, sensitivity, false positive error, undetected target error, and overall agreement indicated E. coli recovery on m-ColiBlue24 was superior to recovery on mTEC for all five parameters. Recovery of total coliforms on the new medium was comparable to recovery on m-Endo.

This article has been cited by other articles:

• Oswald, W. E., Lescano, A. G., Bern, C., Calderon, M. M., Cabrera, L., Gilman, R. H. (2007). Fecal Contamination of Drinking Water within Peri-Urban Households, Lima, Peru. Am J Trop Med Hyg 77: 699-704 [Abstract] [Full Text]  
• Grant, M. A. (2004). Improved Laboratory Enrichment for Enterohemorrhagic Escherichia coli by Exposure to Extremely Acidic Conditions. Appl. Environ. Microbiol. 70: 1226-1230 [Abstract] [Full Text]  
• Chao, K.-K., Chao, C.-C., Chao, W.-L. (2004). Evaluation of Colilert-18 for Detection of Coliforms and Eschericha coli in Subtropical Freshwater. Appl. Environ. Microbiol. 70: 1242-1244 [Abstract] [Full Text]  
• Grant, M. A., Weagant, S. D., Feng, P. (2001). Glutamate Decarboxylase Genes as a Prescreening Marker for Detection of Pathogenic Escherichia coli Groups. Appl. Environ. Microbiol. 67: 3110-3114 [Abstract] [Full Text]