This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jimenez-Salgado, T. Articles by Caballero-Mellado, J. Search for Related Content PubMed PubMed Citation Articles by Jimenez-Salgado, T. Articles by Caballero-Mellado, J. Agricola Articles by Jimenez-Salgado, T. Articles by Caballero-Mellado, J.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 09 1997, 3676-3683, Vol 63, No. 9
Copyright © 1997, American Society for Microbiology

Coffea arabica L., a new host plant for Acetobacter diazotrophicus, and isolation of other nitrogen-fixing acetobacteria

T Jimenez-Salgado, LE Fuentes-Ramirez, A Tapia-Hernandez, MA Mascarua-Esparza, E Martinez-Romero and J Caballero-Mellado
Departamento de Genetica Molecular, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico.

Acetobacter diazotrophicus was isolated from coffee plant tissues and from rhizosphere soils. Isolation frequencies ranged from 15 to 40% and were dependent on soil pH. Attempts to isolate this bacterial species from coffee fruit, from inside vesicular-arbuscular mycorrhizal fungi spores, or from mealybugs (Planococcus citri) associated with coffee plants were not successful. Other acid-producing diazotrophic bacteria were recovered with frequencies of 20% from the coffee rhizosphere. These N2-fixing isolates had some features in common with the genus Acetobacter but should not be assigned to the species Acetobacter diazotrophicus because they differed from A. diazotrophicus in morphological and biochemical traits and were largely divergent in electrophoretic mobility patterns of metabolic enzymes at coefficients of genetic distance as high as 0.950. In addition, these N2-fixing acetobacteria differed in the small-subunit rRNA restriction fragment length polymorphism patterns obtained with EcoRI, and they exhibited very low DNA-DNA homology levels, ranging from 11 to 15% with the A. diazotrophicus reference strain PAI 5T. Thus, some of the diazotrophic acetobacteria recovered from the rhizosphere of coffee plants may be regarded as N2-fixing species of the genus Acetobacter other than A. diazotrophicus. Endophytic diazotrophic bacteria may be more prevalent than previously thought, and perhaps there are many more potentially beneficial N2-fixing bacteria which can be isolated from other agronomically important crops.

This article has been cited by other articles:

• Dutta, D., Gachhui, R. (2007). Nitrogen-fixing and cellulose-producing Gluconacetobacter kombuchae sp. nov., isolated from Kombucha tea. Int. J. Syst. Evol. Microbiol. 57: 353-357 [Abstract] [Full Text]  
• Dutta, D., Gachhui, R. (2006). Novel nitrogen-fixing Acetobacter nitrogenifigens sp. nov., isolated from Kombucha tea.. Int. J. Syst. Evol. Microbiol. 56: 1899-1903 [Abstract] [Full Text]  
• Arrieta, J. G., Sotolongo, M., Menendez, C., Alfonso, D., Trujillo, L. E., Soto, M., Ramirez, R., Hernandez, L. (2004). A Type II Protein Secretory Pathway Required for Levansucrase Secretion by Gluconacetobacter diazotrophicus. J. Bacteriol. 186: 5031-5039 [Abstract] [Full Text]  
• Loganathan, P., Nair, S. (2004). Swaminathania salitolerans gen. nov., sp. nov., a salt-tolerant, nitrogen-fixing and phosphate-solubilizing bacterium from wild rice (Porteresia coarctata Tateoka). Int. J. Syst. Evol. Microbiol. 54: 1185-1190 [Abstract] [Full Text]  
• Perlova, O., Ureta, A., Nordlund, S., Meletzus, D. (2003). Identification of Three Genes Encoding PII-Like Proteins in Gluconacetobacter diazotrophicus: Studies of Their Role(s) in the Control of Nitrogen Fixation. J. Bacteriol. 185: 5854-5861 [Abstract] [Full Text]  
• Ureta, A., Nordlund, S. (2002). Evidence for Conformational Protection of Nitrogenase against Oxygen in Gluconacetobacter diazotrophicus by a Putative FeSII Protein. J. Bacteriol. 184: 5805-5809 [Abstract] [Full Text]  
• Pan, B., Vessey, J. K. (2001). Response of the Endophytic Diazotroph Gluconacetobacter diazotrophicus on Solid Media to Changes in Atmospheric Partial O2 Pressure. Appl. Environ. Microbiol. 67: 4694-4700 [Abstract] [Full Text]  
• Estrada-De Los Santos, P., Bustillos-Cristales, R., Caballero-Mellado, J. (2001). Burkholderia, a Genus Rich in Plant-Associated Nitrogen Fixers with Wide Environmental and Geographic Distribution. Appl. Environ. Microbiol. 67: 2790-2798 [Abstract] [Full Text]  
• Magalhães Cruz, L., Maltempi de Souza, E., Weber, O. B., Baldani, J. I., Döbereiner, J., de Oliveira Pedrosa, F. (2001). 16S Ribosomal DNA Characterization of Nitrogen-Fixing Bacteria Isolated from Banana (Musa spp.) and Pineapple (Ananas comosus (L.) Merril). Appl. Environ. Microbiol. 67: 2375-2379 [Abstract] [Full Text]