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Appl Environ Microbiol, January 1998, p. 231-237, Vol. 64, No. 1
Department of Food Science, Cook College, New
Jersey Agricultural Experiment Station, Rutgers, The State
University of New Jersey, New Brunswick, New Jersey 08901-8520
Received 24 July 1997/Accepted 24 October 1997
Nisin resistance in Listeria monocytogenes ATCC 700302 is a complex phenotype involving alterations in both the cytoplasmic membrane and the cell wall and a requirement for divalent cations. In
addition to a lower ratio of C15 to C17 fatty
acids than in the wild-type strain (A. S. Mazzotta and T. J. Montville, J. Appl. Microbiol. 82:32-38, 1997), this
nisin-resistant (Nisr) strain contained significantly more
zwitterionic phosphatidylethanolamine and less anionic
phosphatidylglycerol and cardiolipin. The extraction of cardiolipin was
enhanced by a penicillin-lysozyme step to disrupt the cell wall. This
study is the first to quantify the phosphatidylethanolamine component of the L. monocytogenes cytoplasmic
membrane. While these cytoplasmic membrane changes were induced by
nisin, the Nisr strain also showed altered sensitivities to
cell wall-acting compounds, even when grown in the absence of nisin,
suggesting a constitutive alteration in the strain's cell wall. A
model which integrates the roles of the cell membrane, cell wall, and
divalent cations is presented. Finally, nisin resistance in
L. monocytogenes ATCC 700302 conferred
cross-resistance to the class IIa bacteriocin pediocin PA-1 and the
class IV leuconocin S.
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Nisin Resistance in Listeria
monocytogenes ATCC 700302 Is a Complex Phenotype
and
*
Corresponding author. Mailing address: Department
of Food Science, Cook College, New Jersey Agricultural Experiment
Station, Rutgers, The State University of New Jersey, 65 Dudley Rd.,
New Brunswick, NJ 08901-8520. Phone: (732) 932-9611, ext. 201. Fax: (732) 932-6776. E-mail: montville{at}aesop.rutgers.edu.
Manuscript D-10974-2-97 of the New Jersey Agricultural Experiment
Station.
Present address: Department of Food Science, Cornell University,
Ithaca, NY 14853.
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