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Appl Environ Microbiol, January 1998, p. 352-355, Vol. 64, No. 1
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of Energy and Growth Yields for Desulfitobacterium dehalogenans during Utilization of Chlorophenol and Various Traditional Electron Acceptors

M. Mackiewicz and J. Wiegel*

Department of Microbiology and Center for Biological Resource Recovery, University of Georgia, Athens, Georgia 30602-2605

Received 4 June 1997/Accepted 13 October 1997

Desulfitobacterium dehalogenans grew with formate as the electron donor and 3-chloro-4-hydroxyphenylacetate (3-Cl-4-OHPA) as the electron acceptor, yielding YX/formate, YX/2e-, and YX/ATP ranging from 3.2 to 11.3 g of biomass (dry weight)/mol, thus indicating that energy was conserved through reductive dechlorination. Pyruvate was utilized as the electron donor and acceptor, yielding stoichiometric amounts of acetate and lactate, respectively, and a YX/reduced acceptor of 13.0 g of biomass (dry weight)/mol. The supplementation of pyruvate-containing medium with additional electron acceptors, such as 3-Cl-4-OHPA, nitrate, fumarate, or sulfite, caused pyruvate to be replaced as the electron acceptor and nearly doubled the YX/ATP (YX/acetate formed). A comparison of the yields for 3-Cl-4-OHPA with those for other traditional electron acceptors indicates that the dehalogenation reaction led to the formation of similar amounts of energy equivalents. The various electron acceptors were used concomitantly with 3-Cl-4-OHPA in nonacclimated cultures, but the utilization rates and amounts utilized differed.

* Corresponding author. Mailing address: Department of Microbiology and Center for Biological Resource Recovery, University of Georgia, Athens, GA 30602-2605. Phone: (706) 542-2651. Fax: (706) 542-2674. E-mail: JWIEGEL{at}uga.cc.uga.edu.




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