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Appl Environ Microbiol, January 1998, p. 363-365, Vol. 64, No. 1
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Differentiation of Campylobacter Isolates on the Basis of Sensitivity to Boiling in Water as Measured by PCR-Detectable DNA

Zaynab S. Mohran,1,* Ray R. Arthur,1,2 Buhari A. Oyofo,1 Leonard F. Peruski,1 Momtaz O. Wasfy,1 Tharwat F. Ismail,1 and James R. Murphy1,3

U.S. Naval Medical Research Unit No. 3, Cairo, Egypt1; School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland 212052; and Center of Infectious Diseases, University of Texas, Houston, Texas3

Received 17 June 1997/Accepted 30 September 1997

Differential sensitivity for the release of PCR-detectable genomic DNA upon boiling in water is reported for 45 Campylobacter jejuni and Campylobacter coli strains isolated in Egypt. All of the strains released PCR-detectable DNA when treated with proteinase K and sodium dodecyl sulfate. When DNA was extracted from these strains by boiling in water, nine (20%) of the strains were PCR negative or resistant to boiling, suggesting the presence of boiling-sensitive and boiling-resistant phenotypes.

* Corresponding author. Mailing address: Research Publications Branch, U.S. Naval Medical Research Unit No. 3, PSC 452, Box 5000, FPO New York, AE 09835-0007. Phone: 011-202-284-1381. Fax: 011-202-284-1382. E-mail: namru3_RSD{at}centcom.dsaa.osd.mil.




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Copyright © 1998 by the American Society for Microbiology. All rights reserved.