This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huber, R.
Right arrow Articles by Stetter, K. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huber, R.
Right arrow Articles by Stetter, K. O.
Agricola
Right arrow Articles by Huber, R.
Right arrow Articles by Stetter, K. O.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 1998, p. 3576-3583, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Thermocrinis ruber gen. nov., sp. nov., a Pink-Filament-Forming Hyperthermophilic Bacterium Isolated from Yellowstone National Park

Robert Huber,1,* Wolfgang Eder,1 Stefan Heldwein,1 Gerhard Wanner,2 Harald Huber,1 Reinhard Rachel,1 and Karl O. Stetter1

Lehrstuhl für Mikrobiologie and Archaeenzentrum, Universität Regensburg, D-93053 Regensburg,1 and Botanisches Institut, Universität München, D-80638 Munich,2 Germany

Received 4 May 1998/Accepted 23 July 1998

A novel hyperthermophilic bacterium was isolated from pink filamentous streamers (pink filaments) occurring in the upper outflow channel (temperature, 82 to 88°C) of Octopus Spring in Yellowstone National Park, Wyo. The gram-negative cells grew at low salinity at temperatures up to 89°C in the neutral to alkaline pH range. Depending on the culture conditions, the organisms occurred as single motile rods, as aggregates, or as long filaments that formed streamer-like cell masses. The novel isolate grew chemolithoautotrophically with hydrogen, thiosulfate, and elemental sulfur as electron donors and oxygen as the electron acceptor. Alternatively, under aerobic conditions, formate and formamide served as sole energy and carbon sources. The novel isolate had a 16S rRNA sequence closely related to the 16S rRNA sequence obtained from uncultivated pink filaments. It represents a new genus in the order Aquificales, the type species of which we name Thermocrinis ruber (type strain, OC 1/4 [= DSM 12173]).


* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie, Universität Regensburg, Universitätsstr. 31, 93053 Regensburg, Germany. Phone: 0941/943-3182. Fax: 0941/943-2403. E-mail: Robert.Huber{at}Biologie.Uni-Regensburg.de.


Applied and Environmental Microbiology, October 1998, p. 3576-3583, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Mori, K., Suzuki, K.-i. (2008). Thiofaba tepidiphila gen. nov., sp. nov., a novel obligately chemolithoautotrophic, sulfur-oxidizing bacterium of the Gammaproteobacteria isolated from a hot spring. Int. J. Syst. Evol. Microbiol. 58: 1885-1891 [Abstract] [Full Text]  
  • Hall, J. R., Mitchell, K. R., Jackson-Weaver, O., Kooser, A. S., Cron, B. R., Crossey, L. J., Takacs-Vesbach, C. D. (2008). Molecular Characterization of the Diversity and Distribution of a Thermal Spring Microbial Community by Using rRNA and Metabolic Genes. Appl. Environ. Microbiol. 74: 4910-4922 [Abstract] [Full Text]  
  • Antunes, A., Rainey, F. A., Wanner, G., Taborda, M., Patzold, J., Nobre, M. F., da Costa, M. S., Huber, R. (2008). A New Lineage of Halophilic, Wall-Less, Contractile Bacteria from a Brine-Filled Deep of the Red Sea. J. Bacteriol. 190: 3580-3587 [Abstract] [Full Text]  
  • Flores, G. E., Liu, Y., Ferrera, I., Beveridge, T. J., Reysenbach, A.-L. (2008). Sulfurihydrogenibium kristjanssonii sp. nov., a hydrogen- and sulfur-oxidizing thermophile isolated from a terrestrial Icelandic hot spring. Int. J. Syst. Evol. Microbiol. 58: 1153-1158 [Abstract] [Full Text]  
  • Nunoura, T., Miyazaki, M., Suzuki, Y., Takai, K., Horikoshi, K. (2008). Hydrogenivirga okinawensis sp. nov., a thermophilic sulfur-oxidizing chemolithoautotroph isolated from a deep-sea hydrothermal field, Southern Okinawa Trough. Int. J. Syst. Evol. Microbiol. 58: 676-681 [Abstract] [Full Text]  
  • Hetzer, A., McDonald, I. R., Morgan, H. W. (2008). Venenivibrio stagnispumantis gen. nov., sp. nov., a thermophilic hydrogen-oxidizing bacterium isolated from Champagne Pool, Waiotapu, New Zealand. Int. J. Syst. Evol. Microbiol. 58: 398-403 [Abstract] [Full Text]  
  • Weber, S. D., Ludwig, W., Schleifer, K.-H., Fried, J. (2007). Microbial Composition and Structure of Aerobic Granular Sewage Biofilms. Appl. Environ. Microbiol. 73: 6233-6240 [Abstract] [Full Text]  
  • Mathur, J., Bizzoco, R. W., Ellis, D. G., Lipson, D. A., Poole, A. W., Levine, R., Kelley, S. T. (2007). Effects of Abiotic Factors on the Phylogenetic Diversity of Bacterial Communities in Acidic Thermal Springs. Appl. Environ. Microbiol. 73: 2612-2623 [Abstract] [Full Text]  
  • L'Haridon, S., Reysenbach, A.-L., Tindall, B. J., Schonheit, P., Banta, A., Johnsen, U., Schumann, P., Gambacorta, A., Stackebrandt, E., Jeanthon, C. (2006). Desulfurobacterium atlanticum sp. nov., Desulfurobacterium pacificum sp. nov. and Thermovibrio guaymasensis sp. nov., three thermophilic members of the Desulfurobacteriaceae fam. nov., a deep branching lineage within the Bacteria. Int. J. Syst. Evol. Microbiol. 56: 2843-2852 [Abstract] [Full Text]  
  • Griffiths, E., Gupta, R. S. (2006). Molecular signatures in protein sequences that are characteristics of the phylum Aquificae. Int. J. Syst. Evol. Microbiol. 56: 99-107 [Abstract] [Full Text]  
  • Spear, J. R., Walker, J. J., McCollom, T. M., Pace, N. R. (2005). From The Cover: Hydrogen and bioenergetics in the Yellowstone geothermal ecosystem. Proc. Natl. Acad. Sci. USA 102: 2555-2560 [Abstract] [Full Text]  
  • Hirayama, H., Takai, K., Inagaki, F., Nealson, K. H., Horikoshi, K. (2005). Thiobacter subterraneus gen. nov., sp. nov., an obligately chemolithoautotrophic, thermophilic, sulfur-oxidizing bacterium from a subsurface hot aquifer. Int. J. Syst. Evol. Microbiol. 55: 467-472 [Abstract] [Full Text]  
  • Nakagawa, S., Nakamura, S., Inagaki, F., Takai, K., Shirai, N., Sako, Y. (2004). Hydrogenivirga caldilitoris gen. nov., sp. nov., a novel extremely thermophilic, hydrogen- and sulfur-oxidizing bacterium from a coastal hydrothermal field. Int. J. Syst. Evol. Microbiol. 54: 2079-2084 [Abstract] [Full Text]  
  • Aguiar, P., Beveridge, T. J., Reysenbach, A.-L. (2004). Sulfurihydrogenibium azorense, sp. nov., a thermophilic hydrogen-oxidizing microaerophile from terrestrial hot springs in the Azores. Int. J. Syst. Evol. Microbiol. 54: 33-39 [Abstract] [Full Text]  
  • Nakagawa, T., Fukui, M. (2003). Molecular Characterization of Community Structures and Sulfur Metabolism within Microbial Streamers in Japanese Hot Springs. Appl. Environ. Microbiol. 69: 7044-7057 [Abstract] [Full Text]  
  • Nakagawa, S., Takai, K., Horikoshi, K., Sako, Y. (2003). Persephonella hydrogeniphila sp. nov., a novel thermophilic, hydrogen-oxidizing bacterium from a deep-sea hydrothermal vent chimney. Int. J. Syst. Evol. Microbiol. 53: 863-869 [Abstract] [Full Text]  
  • Blank, C. E., Cady, S. L., Pace, N. R. (2002). Microbial Composition of Near-Boiling Silica-Depositing Thermal Springs throughout Yellowstone National Park. Appl. Environ. Microbiol. 68: 5123-5135 [Abstract] [Full Text]  
  • Takai, K., Hirayama, H., Sakihama, Y., Inagaki, F., Yamato, Y., Horikoshi, K. (2002). Isolation and Metabolic Characteristics of Previously Uncultured Members of the Order Aquificales in a Subsurface Gold Mine. Appl. Environ. Microbiol. 68: 3046-3054 [Abstract] [Full Text]  
  • Reysenbach, A.-L., Shock, E. (2002). Merging Genomes with Geochemistry in Hydrothermal Ecosystems. Science 296: 1077-1082 [Abstract] [Full Text]  
  • Jahnke, L. L., Eder, W., Huber, R., Hope, J. M., Hinrichs, K.-U., Hayes, J. M., Des Marais, D. J., Cady, S. L., Summons, R. E. (2001). Signature Lipids and Stable Carbon Isotope Analyses of Octopus Spring Hyperthermophilic Communities Compared with Those of Aquificales Representatives. Appl. Environ. Microbiol. 67: 5179-5189 [Abstract] [Full Text]  
  • Eder, W., Jahnke, L. L., Schmidt, M., Huber, R. (2001). Microbial Diversity of the Brine-Seawater Interface of the Kebrit Deep, Red Sea, Studied via 16S rRNA Gene Sequences and Cultivation Methods. Appl. Environ. Microbiol. 67: 3077-3085 [Abstract] [Full Text]  
  • Rudolph, C., Wanner, G., Huber, R. (2001). Natural Communities of Novel Archaea and Bacteria Growing in Cold Sulfurous Springs with a String-of-Pearls-Like Morphology. Appl. Environ. Microbiol. 67: 2336-2344 [Abstract] [Full Text]  
  • Vieille, C., Zeikus, G. J. (2001). Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability. Microbiol. Mol. Biol. Rev. 65: 1-43 [Abstract] [Full Text]  
  • Skirnisdottir, S., Hreggvidsson, G. O., Hjörleifsdottir, S., Marteinsson, V. T., Petursdottir, S. K., Holst, O., Kristjansson, J. K. (2000). Influence of Sulfide and Temperature on Species Composition and Community Structure of Hot Spring Microbial Mats. Appl. Environ. Microbiol. 66: 2835-2841 [Abstract] [Full Text]  
  • Takai, K., Horikoshi, K. (1999). Genetic Diversity of Archaea in Deep-Sea Hydrothermal Vent Environments. Genetics 152: 1285-1297 [Abstract] [Full Text]  
  • Ward, D. M., Ferris, M. J., Nold, S. C., Bateson, M. M. (1998). A Natural View of Microbial Biodiversity within Hot Spring Cyanobacterial Mat Communities. Microbiol. Mol. Biol. Rev. 62: 1353-1370 [Abstract] [Full Text]