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Applied and Environmental Microbiology, October 1998, p. 3972-3976, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Enzymatic Conversion of Glucose to UDP-4-Keto-6-Deoxyglucose in Streptomyces spp.

Song Yu Liudagger and John P. N. Rosazza*

Division of Medicinal and Natural Products Chemistry and Center for Biocatalysis and Bioprocessing, College of Pharmacy, The University of Iowa, Iowa City, Iowa 52242

Received 17 April 1998/Accepted 10 August 1998

All of the 2,6-dideoxy sugars contained within the structure of chromomycin A3 are derived from D-glucose. Enzyme assays were used to confirm the presence of hexokinase, phosphoglucomutase, UDPG pyrophosphorylase (UDPGP), and UDPG oxidoreductase (UDPGO), all of which are involved in the pathway of glucose activation and conversion into 2,6-dideoxyhexoses during chromomycin biosynthesis. Levels of the four enzymes in Streptomyces spp. cell extracts were correlated with the production of chromomycins. The pathway of sugar activation in Streptomyces spp. involves glucose 6-phosphorylation by hexokinase, isomerization to G-1-P catalyzed by phosphoglucomutase, synthesis of UDPG catalyzed by UDPGP, and formation of UDP-4-keto-6-deoxyglucose by UDPGO.

* Corresponding author. Mailing address: Division of Medicinal and Natural Products Chemistry and Center for Biocatalysis and Bioprocessing, College of Pharmacy, The University of Iowa, Iowa City, IA 52242. Phone: (319) 335-4902. Fax: (319) 335-4901. E-mail: johnrosazza{at}uiowa.edu.

dagger Present address: Roche Colorado Corporation, Boulder Technology Center, Boulder, CO 80301-2880.

Applied and Environmental Microbiology, October 1998, p. 3972-3976, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.