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Applied and Environmental Microbiology, October 1998, p. 4068-4072, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Development of Bacterium-Based Heavy Metal Biosorbents: Enhanced Uptake of Cadmium and Mercury by Escherichia coli Expressing a Metal Binding Motif

Mehran Pazirandeh,* Bridget M. Wells, and Rebecca L. Ryan

Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, D.C. 20375

Received 24 April 1998/Accepted 10 July 1998

A gene coding for a de novo peptide sequence containing a metal binding motif was chemically synthesized and expressed in Escherichia coli as a fusion with the maltose binding protein. Bacterial cells expressing the metal binding peptide fusion demonstrated enhanced binding of Cd2+ and Hg2+ compared to bacterial cells lacking the metal binding peptide. The potential use of genetically engineered bacteria as biosorbents for the removal of heavy metals from wastewaters is discussed.

* Corresponding author. Mailing address: Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375. Phone: (202) 404-6073. Fax: (202) 767-9594. E-mail: mpp{at}cbmse.nrl.navy.mil.

Applied and Environmental Microbiology, October 1998, p. 4068-4072, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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