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Applied and Environmental Microbiology, October 1998, p. 4073-4075, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Purification and Characterization of an NAD-Malic Enzyme from Bradyrhizobium japonicum A1017

Fan Chen, Youichi Okabe, Kaoru Osano, and Shigeyuki Tajima*

Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Ikenobe 2393, Kita-gun, Kagawa 761-07, Japan

Received 17 March 1997/Accepted 12 July 1998

An NAD-malic enzyme was purified to homogeneity from Bradyrhizobium japonicum A1017, and its molecular characteristics were surveyed. The enzyme exhibited native and subunit molecular masses of 388 and 85 kDa, respectively, suggesting that it exists as a homotetramer, and was activated by metabolic intermediates in glycolysis. The role of the enzyme in bacteroids' carbon metabolism is discussed.

* Corresponding author. Mailing address: Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Ikenobe 2393, Miki-cho, Kita-gun, Kagawa 761-07, Japan. Phone: (0878)98-9620. Fax: (0878)98-7295. E-mail: tajima{at}ag.kagawa-u.ac.jp.

Applied and Environmental Microbiology, October 1998, p. 4073-4075, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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