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Applied and Environmental Microbiology, October 1998, p. 4098-4102, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Loss of Ammonia Monooxygenase Activity in Nitrosomonas europaea upon Exposure to Nitrite

Lisa Y. Stein and Daniel J. Arp*

Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331

Received 16 April 1998/Accepted 28 July 1998

Nitrosomonas europaea, an obligate ammonia-oxidizing bacterium, lost an increasing amount of ammonia oxidation activity upon exposure to increasing concentrations of nitrite, the primary product of ammonia-oxidizing metabolism. The loss of activity was specific to the ammonia monooxygenase (AMO) enzyme, as confirmed by a decreased rate of NH4+-dependent O2 consumption, some loss of active AMO molecules observed by polypeptide labeling with 14C2H2, the protection of activity by substrates of AMO, and the requirement for copper. The loss of AMO activity via nitrite occurred under both aerobic and anaerobic conditions, and more activity was lost under alkaline than under acidic conditions except in the presence of large concentrations (20 mM) of nitrite. These results indicate that nitrite toxicity in N. europaea is mediated by a unique mechanism that is specific for AMO.

* Corresponding author. Mailing address: Department of Botany and Plant Pathology, 2082 Cordley, Oregon State University, Corvallis, OR 97331. Phone: (541) 737-1294. Fax: (541) 737-3573. E-mail: arpd{at}bcc.orst.edu.

Applied and Environmental Microbiology, October 1998, p. 4098-4102, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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