This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kreader, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kreader, C. A.
Agricola
Right arrow Articles by Kreader, C. A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 1998, p. 4103-4105, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Persistence of PCR-Detectable Bacteroides distasonis from Human Feces in River Water

Carol A. Kreader*

Microbiological and Chemical Exposure Assessment Research Division, National Exposure Research Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio

Received 16 April 1998/Accepted 24 July 1998

To evaluate the persistence of PCR-detectable Bacteroides distasonis in surface water, whole human feces were dispersed into water from the Ohio River and incubated in flasks in the laboratory or in diffusion chambers in situ. Duplicate samples were taken daily, and material that pelleted at 16,000 × g was assayed by PCR. Persistence of PCR-detectable DNA from this anaerobe depended upon temperature and predation, two of the factors shown by others to influence the survival of aerobic bacteria detected by culture. B. distasonis was detected by PCR for at least 2 weeks at 4°C but for only 4 to 5 days at 14°C, 1 to 2 days at 24°C, and 1 day at 30°C. In filtered water or in the presence of cycloheximide, a eukaryotic inhibitor, persistence at 24°C was extended by at least a week.

* Present address: Sigma Chemical Company, Molecular R&D, N-bldg., 3300 S. 2nd St., St. Louis, MO 63118. Phone: (800) 521-8956, ext. 8766. Fax: (314) 286-7645. E-mail: ckreader{at}notesgw.sial.com.

Applied and Environmental Microbiology, October 1998, p. 4103-4105, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bell, A., Layton, A. C., McKay, L., Williams, D., Gentry, R., Sayler, G. S. (2009). Factors Influencing the Persistence of Fecal Bacteroides in Stream Water. J. Environ. Qual. 38: 1224-1232 [Abstract] [Full Text]  
  • Lee, Y.-J., Molina, M., Santo Domingo, J. W., Willis, J. D., Cyterski, M., Endale, D. M., Shanks, O. C. (2008). Temporal Assessment of the Impact of Exposure to Cow Feces in Two Watersheds by Multiple Host-Specific PCR Assays. Appl. Environ. Microbiol. 74: 6839-6847 [Abstract] [Full Text]  
  • Gourmelon, M., Caprais, M. P., Segura, R., Le Mennec, C., Lozach, S., Piriou, J. Y., Rince, A. (2007). Evaluation of Two Library-Independent Microbial Source Tracking Methods To Identify Sources of Fecal Contamination in French Estuaries. Appl. Environ. Microbiol. 73: 4857-4866 [Abstract] [Full Text]  
  • McQuaig, S. M., Scott, T. M., Harwood, V. J., Farrah, S. R., Lukasik, J. O. (2006). Detection of Human-Derived Fecal Pollution in Environmental Waters by Use of a PCR-Based Human Polyomavirus Assay. Appl. Environ. Microbiol. 72: 7567-7574 [Abstract] [Full Text]  
  • Reischer, G. H., Kasper, D. C., Steinborn, R., Mach, R. L., Farnleitner, A. H. (2006). Quantitative PCR Method for Sensitive Detection of Ruminant Fecal Pollution in Freshwater and Evaluation of This Method in Alpine Karstic Regions. Appl. Environ. Microbiol. 72: 5610-5614 [Abstract] [Full Text]  
  • Walters, S. P., Field, K. G. (2006). Persistence and growth of fecal bacteroidales assessed by bromodeoxyuridine immunocapture.. Appl. Environ. Microbiol. 72: 4532-4539 [Abstract] [Full Text]  
  • Layton, A., McKay, L., Williams, D., Garrett, V., Gentry, R., Sayler, G. (2006). Development of Bacteroides 16S rRNA Gene TaqMan-Based Real-Time PCR Assays for Estimation of Total, Human, and Bovine Fecal Pollution in Water.. Appl. Environ. Microbiol. 72: 4214-4224 [Abstract] [Full Text]  
  • Bower, P. A., Scopel, C. O., Jensen, E. T., Depas, M. M., McLellan, S. L. (2005). Detection of Genetic Markers of Fecal Indicator Bacteria in Lake Michigan and Determination of Their Relationship to Escherichia coli Densities Using Standard Microbiological Methods. Appl. Environ. Microbiol. 71: 8305-8313 [Abstract] [Full Text]  
  • Fogarty, L. R., Voytek, M. A. (2005). Comparison of Bacteroides-Prevotella 16S rRNA Genetic Markers for Fecal Samples from Different Animal Species. Appl. Environ. Microbiol. 71: 5999-6007 [Abstract] [Full Text]  
  • Bernhard, A. E., Field, K. G. (2000). Identification of Nonpoint Sources of Fecal Pollution in Coastal Waters by Using Host-Specific 16S Ribosomal DNA Genetic Markers from Fecal Anaerobes. Appl. Environ. Microbiol. 66: 1587-1594 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»