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Applied and Environmental Microbiology, November 1998, p. 4246-4254, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

PCR-Ribotyping of Xenorhabdus and Photorhabdus Isolates from the Caribbean Region in Relation to the Taxonomy and Geographic Distribution of Their Nematode Hosts

Marion Fischer-Le Saux,1 Hervé Mauléon,2 Philippe Constant,2 Brigitte Brunel,3 and Noël Boemare1,*

Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Laboratoire de Pathologie Comparée, Université Montpellier II, F-34095 Montpellier Cedex 5,1 Institut National de la Recherche Agronomique, Unité de Recherches en Productions Végétales, Domaine Duclos, F-97165 Pointe-à-Pitre Cedex,2 and École Nationale Supérieure Agronomique de Montpellier, Institut National de la Recherche Agronomique, Laboratoire de Recherche sur les Symbiotes des Racines and Unité de Recherches et de Formation des Sciences du Sol, F-34 060 Montpellier Cedex 1,3 France

Received 24 February 1998/Accepted 24 August 1998

The genetic diversity of symbiotic Xenorhabdus and Photorhabdus bacteria associated with entomopathogenic nematodes was examined by a restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes (rDNAs). A total of 117 strains were studied, most of which were isolated from the Caribbean basin after an exhaustive soil sampling. The collection consisted of 77 isolates recovered from entomopathogenic nematodes in 14 Caribbean islands and of 40 reference strains belonging to Xenorhabdus and Photorhabdus spp. collected at various localities worldwide. Thirty distinctive 16S rDNA genotypes were identified, and cluster analysis was used to distinguish the genus Xenorhabdus from the genus Photorhabdus. The genus Xenorhabdus appears more diverse than the genus Photorhabdus, and for both genera the bacterial genotype diversity is in congruence with the host-nematode taxonomy. The occurrence of symbiotic bacterial genotypes was related to the ecological distribution of host nematodes.

* Corresponding author. Mailing address: Laboratoire de Pathologie Comparée, C.P. 101, Université Montpellier II, 34095 Montpellier Cedex 5, France. Phone: 33-4-67143740. Fax: 33-4-67144679. E-mail: boemare{at}ensam.inra.fr.

Applied and Environmental Microbiology, November 1998, p. 4246-4254, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cowles, C. E., Goodrich-Blair, H. (2008). The Xenorhabdus nematophila nilABC Genes Confer the Ability of Xenorhabdus spp. To Colonize Steinernema carpocapsae Nematodes. J. Bacteriol. 190: 4121-4128 [Abstract] [Full Text]  
  • Tailliez, P., Pages, S., Ginibre, N., Boemare, N. (2006). New insight into diversity in the genus Xenorhabdus, including the description of ten novel species. Int. J. Syst. Evol. Microbiol. 56: 2805-2818 [Abstract] [Full Text]  
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