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Applied and Environmental Microbiology, November 1998, p. 4255-4259, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Monoclonal Antibodies Raised against Native Major Capsid Proteins of Lactococcal c2-Like Bacteriophages

Sandra R. Chibani Azaïez,1,2 Ismaïl Fliss,2 Ronald E. Simard,2 and Sylvain Moineau1,*

Department of Biochemistry and Groupe de Recherche en Ecologie Buccale (GREB), Faculté de Médecine Dentaire,1 and Department of Food Science and Nutrition, Centre de Recherche en Sciences et Technologie du Lait (STELA),2 Université Laval, Québec, Canada G1K 7P4

Received 23 March 1998/Accepted 11 August 1998

Phage Q38, a representative member of the c2 species, was purified by CsCl gradient and used to immunize BALB/c mice. Monoclonal antibodies (MAbs) were raised and then characterized by enzyme-linked immunosorbent assay. Two MAbs of isotype immunoglobulin G2a, designated 2A5 and 6G7, reacted only with phages belonging to the c2 species and not with phages of the 936 and P335 species, with a Lactococcus lactis cell extract, or with phage DNA. Immunoelectron microscopy showed that both MAbs recognized only phage head proteins. They did not react with any denatured phage proteins in Western blot assays. However, when the nitrocellulose membranes were treated with a Triton-based buffer to assist in protein renaturation, MAbs 2A5 and 6G7 recognized the two major capsid proteins with molecular masses of 80 and 170 kDa. Competitive inhibition tests showed that the two MAbs bind to overlapping epitopes. These MAbs may be a useful tool for monitoring c2 bacteriophages during dairy fermentation and in genetic studies.

* Corresponding author. Mailing address: Université Laval, Dept. Biochimie, Pavillon Vachon, Québec, Canada G1K 7P4. Phone: (418) 656-3712. Fax: (418) 656-2861. E-mail: Sylvain.Moineau{at}bcm.ulaval.ca.

Applied and Environmental Microbiology, November 1998, p. 4255-4259, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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