Seasonal Community and Population Dynamics of Pelagic Bacteria and Archaea in a High Mountain Lake

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Pernthaler, J.
	Articles by Amann, R.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Pernthaler, J.
	Articles by Amann, R.


Agricola

	Articles by Pernthaler, J.
	Articles by Amann, R.

Previous Article | Next Article

Applied and Environmental Microbiology, November 1998, p. 4299-4306, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Seasonal Community and Population Dynamics of Pelagic Bacteria and Archaea in a High Mountain Lake

Jakob Pernthaler,¹^,^* Frank-Oliver Glöckner,¹ Stefanie Unterholzner,² Albin Alfreider,² Roland Psenner,² and Rudolf Amann¹

Max-Planck-Institut für marine Mikrobiologie, D-28359 Bremen, Germany,¹ and Institut für Zoologie und Limnologie, University of Innsbruck, Innsbruck, Austria²

Received 13 May 1998/Accepted 2 September 1998

The seasonal variations in community structure and cell morphology of pelagic procaryotes from a high mountain lake (Gossenköllesee,Austria) were studied by in situ hybridization with rRNA-targetedfluorescently labeled oligonucleotide probes (FISH) and image-analyzedmicroscopy. Compositional changes and biomass fluctuations withinthe assemblage were observed both in summer and beneath the winterice cover and are discussed in the context of physicochemicaland biotic parameters. Proteobacteria of the beta subclass (beta-proteobacteria)formed a dominant fraction of the bacterioplankton (annual mean,24% of the total counts), whereas alpha-proteobacteria were ofsimilar relative importance only during spring (mean, 11%). Bacteriaof the Cytophaga-Flavobacterium cluster, although less abundant,constituted the largest fraction of the filamentous morphotypesduring most of the year, thus contributing significantly to thetotal microbial biomass. Successive peaks of threadlike and rod-shapedarchaea were observed during autumn thermal mixing and the periodof ice cover formation, respectively. A set of oligonucleotideprobes targeted to single phylotypes was constructed from 16SrRNA-encoding gene clone sequences. Three distinct populationsof uncultivated microbes, affiliated with the alpha- and beta-proteobacteria,were subsequently monitored by FISH. About one-quarter of allof the beta-proteobacteria (range, 6 to 53%) could be assignedto only two phylotypes. The bacterial populations studied wereannually recurrent, seasonally variable, and vertically stratified,except during the periods of lake overturn. Their variabilityclearly exceeded the fluctuations of the total microbial assemblage,suggesting that the apparent stability of total bacterioplanktonabundances may mask highly dynamic communityfluctuations.

^* Corresponding author. Mailing address: Max Planck Institute for Marine Microbiology, Celsiusstr. 1, D-28359 Bremen, Germany.Phone: 49 421 2028 940. Fax: 49 421 2028.580. E-mail: jperntha{at}mpi-bremen.de.

Applied and Environmental Microbiology, November 1998, p. 4299-4306, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Percent, S. F., Frischer, M. E., Vescio, P. A., Duffy, E. B., Milano, V., McLellan, M., Stevens, B. M., Boylen, C. W., Nierzwicki-Bauer, S. A. (2008). Bacterial Community Structure of Acid-Impacted Lakes: What Controls Diversity?. Appl. Environ. Microbiol. 74: 1856-1868 [Abstract] [Full Text]
Eiler, A., Bertilsson, S. (2007). Flavobacteria Blooms in Four Eutrophic Lakes: Linking Population Dynamics of Freshwater Bacterioplankton to Resource Availability. Appl. Environ. Microbiol. 73: 3511-3518 [Abstract] [Full Text]
Wu, Q. L., Zwart, G., Schauer, M., Kamst-van Agterveld, M. P., Hahn, M. W. (2006). Bacterioplankton Community Composition along a Salinity Gradient of Sixteen High-Mountain Lakes Located on the Tibetan Plateau, China. Appl. Environ. Microbiol. 72: 5478-5485 [Abstract] [Full Text]
Medina-Sanchez, J. M., Felip, M., Casamayor, E. O. (2005). Catalyzed Reported Deposition-Fluorescence In Situ Hybridization Protocol To Evaluate Phagotrophy in Mixotrophic Protists. Appl. Environ. Microbiol. 71: 7321-7326 [Abstract] [Full Text]
Warnecke, F., Sommaruga, R., Sekar, R., Hofer, J. S., Pernthaler, J. (2005). Abundances, Identity, and Growth State of Actinobacteria in Mountain Lakes of Different UV Transparency. Appl. Environ. Microbiol. 71: 5551-5559 [Abstract] [Full Text]
Pernthaler, J., Amann, R. (2005). Fate of Heterotrophic Microbes in Pelagic Habitats: Focus on Populations. Microbiol. Mol. Biol. Rev. 69: 440-461 [Abstract] [Full Text]
Besemer, K., Moeseneder, M. M., Arrieta, J. M., Herndl, G. J., Peduzzi, P. (2005). Complexity of Bacterial Communities in a River-Floodplain System (Danube, Austria). Appl. Environ. Microbiol. 71: 609-620 [Abstract] [Full Text]
Yannarell, A. C., Triplett, E. W. (2005). Geographic and Environmental Sources of Variation in Lake Bacterial Community Composition. Appl. Environ. Microbiol. 71: 227-239 [Abstract] [Full Text]
Page, K. A., Connon, S. A., Giovannoni, S. J. (2004). Representative Freshwater Bacterioplankton Isolated from Crater Lake, Oregon. Appl. Environ. Microbiol. 70: 6542-6550 [Abstract] [Full Text]
Pernthaler, J., Zollner, E., Warnecke, F., Jurgens, K. (2004). Bloom of Filamentous Bacteria in a Mesotrophic Lake: Identity and Potential Controlling Mechanism. Appl. Environ. Microbiol. 70: 6272-6281 [Abstract] [Full Text]
Jaspers, E., Overmann, J. (2004). Ecological Significance of Microdiversity: Identical 16S rRNA Gene Sequences Can Be Found in Bacteria with Highly Divergent Genomes and Ecophysiologies. Appl. Environ. Microbiol. 70: 4831-4839 [Abstract] [Full Text]
(2004). Crenarchaeotal membrane lipids in lake sediments: A new paleotemperature proxy for continental paleoclimate reconstruction?. Geology 32: 613-616
Feris, K. P., Ramsey, P. W., Frazar, C., Rillig, M., Moore, J. N., Gannon, J. E., Holben, W. E. (2004). Seasonal Dynamics of Shallow-Hyporheic-Zone Microbial Community Structure along a Heavy-Metal Contamination Gradient. Appl. Environ. Microbiol. 70: 2323-2331 [Abstract] [Full Text]
Pernthaler, J., Pernthaler, A., Amann, R. (2003). Automated Enumeration of Groups of Marine Picoplankton after Fluorescence In Situ Hybridization. Appl. Environ. Microbiol. 69: 2631-2637 [Abstract] [Full Text]
Sekar, R., Pernthaler, A., Pernthaler, J., Warnecke, F., Posch, T., Amann, R. (2003). An Improved Protocol for Quantification of Freshwater Actinobacteria by Fluorescence In Situ Hybridization. Appl. Environ. Microbiol. 69: 2928-2935 [Abstract] [Full Text]
Wolf, A., Wiese, J., Jost, G., Witzel, K.-P. (2003). Wide Geographic Distribution of Bacteriophages That Lyse the Same Indigenous Freshwater Isolate (Sphingomonas sp. Strain B18). Appl. Environ. Microbiol. 69: 2395-2398 [Abstract] [Full Text]
Muylaert, K., Van der Gucht, K., Vloemans, N., Meester, L. D., Gillis, M., Vyverman, W. (2002). Relationship between Bacterial Community Composition and Bottom-Up versus Top-Down Variables in Four Eutrophic Shallow Lakes. Appl. Environ. Microbiol. 68: 4740-4750 [Abstract] [Full Text]
Pernthaler, A., Preston, C. M., Pernthaler, J., DeLong, E. F., Amann, R. (2002). Comparison of Fluorescently Labeled Oligonucleotide and Polynucleotide Probes for the Detection of Pelagic Marine Bacteria and Archaea. Appl. Environ. Microbiol. 68: 661-667 [Abstract] [Full Text]
O'Sullivan, L. A., Weightman, A. J., Fry, J. C. (2002). New Degenerate Cytophaga-Flexibacter-Bacteroides-Specific 16S Ribosomal DNA-Targeted Oligonucleotide Probes Reveal High Bacterial Diversity in River Taff Epilithon. Appl. Environ. Microbiol. 68: 201-210 [Abstract] [Full Text]
Eilers, H., Pernthaler, J., Peplies, J., Glockner, F. O., Gerdts, G., Amann, R. (2001). Isolation of Novel Pelagic Bacteria from the German Bight and Their Seasonal Contributions to Surface Picoplankton. Appl. Environ. Microbiol. 67: 5134-5142 [Abstract] [Full Text]
Simek, K., Pernthaler, J., Weinbauer, M. G., Hornák, K., Dolan, J. R., Nedoma, J., Masín, M., Amann, R. (2001). Changes in Bacterial Community Composition and Dynamics and Viral Mortality Rates Associated with Enhanced Flagellate Grazing in a Mesoeutrophic Reservoir. Appl. Environ. Microbiol. 67: 2723-2733 [Abstract] [Full Text]
Battin, T. J., Wille, A., Sattler, B., Psenner, R. (2001). Phylogenetic and Functional Heterogeneity of Sediment Biofilms along Environmental Gradients in a Glacial Stream. Appl. Environ. Microbiol. 67: 799-807 [Abstract] [Full Text]
Bagwell, C. E., Lovell, C. R. (2000). Persistence of Selected Spartina alterniflora Rhizoplane Diazotrophs Exposed to Natural and Manipulated Environmental Variability. Appl. Environ. Microbiol. 66: 4625-4633 [Abstract] [Full Text]
Glöckner, F. O., Zaichikov, E., Belkova, N., Denissova, L., Pernthaler, J., Pernthaler, A., Amann, R. (2000). Comparative 16S rRNA Analysis of Lake Bacterioplankton Reveals Globally Distributed Phylogenetic Clusters Including an Abundant Group of Actinobacteria. Appl. Environ. Microbiol. 66: 5053-5065 [Abstract] [Full Text]
Maruyama, A., Sunamura, M. (2000). Simultaneous Direct Counting of Total and Specific Microbial Cells in Seawater, Using a Deep-Sea Microbe as Target. Appl. Environ. Microbiol. 66: 2211-2215 [Abstract] [Full Text]
DeLong, E. F., Taylor, L. T., Marsh, T. L., Preston, C. M. (1999). Visualization and Enumeration of Marine Planktonic Archaea and Bacteria by Using Polyribonucleotide Probes and Fluorescent In Situ Hybridization. Appl. Environ. Microbiol. 65: 5554-5563 [Abstract] [Full Text]
Glöckner, F. O., Fuchs, B. M., Amann, R. (1999). Bacterioplankton Compositions of Lakes and Oceans: a First Comparison Based on Fluorescence In Situ Hybridization. Appl. Environ. Microbiol. 65: 3721-3726 [Abstract] [Full Text]
Höfle, M. G., Haas, H., Dominik, K. (1999). Seasonal Dynamics of Bacterioplankton Community Structure in a Eutrophic Lake as Determined by 5S rRNA Analysis. Appl. Environ. Microbiol. 65: 3164-3174 [Abstract] [Full Text]
Jürgens, K., Pernthaler, J., Schalla, S., Amann, R. (1999). Morphological and Compositional Changes in a Planktonic Bacterial Community in Response to Enhanced Protozoan Grazing. Appl. Environ. Microbiol. 65: 1241-1250 [Abstract] [Full Text]

J. Bacteriol.	Microbiol. Mol. Biol. Rev.	Eukaryot. Cell	All ASM Journals