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Applied and Environmental Microbiology, November 1998, p. 4317-4320, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Metal Accumulation and Vanadium-Induced Multidrug Resistance by Environmental Isolates of Escherichia hermannii and Enterobacter cloacae

Alicia Hernández, Rafael P. Mellado,* and José L. Martínez

Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología (CSIC), Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain

Received 29 June 1998/Accepted 9 August 1998

Contaminated soils from an oil refinery were screened for the presence of microorganisms capable of accumulating either nickel, vanadium, or both metals. Three strains of bacteria that belonged to the family Enterobacteriaceae were selected. Two of them were Escherichia hermannii strains, and outer membrane profile (OMP) analysis showed that they were similar to a strain of clinical origin; the other one was an Enterobacter cloacae strain that differed from clinical isolates. The selected bacteria accumulated both nickel and vanadium. Growth in the presence of vanadium induced multidrug resistance phenotypes in E. hermannii and E. cloacae. Incubation with this metal changed the OMP profile of E. hermannii but did not produce variations in the expression of the major OMPs of E. cloacae.

* Corresponding author. Mailing address: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología (CSIC), Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain. Phone: 34-91-5854547. Fax: 34-91-5854506. E-mail: rpmellado{at}cnb.uam.es.

Applied and Environmental Microbiology, November 1998, p. 4317-4320, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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