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Applied and Environmental Microbiology, November 1998, p. 4353-4356, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Whole-Cell Kinetics of Trichloroethylene Degradation by Phenol Hydroxylase in a Ralstonia eutropha JMP134 Derivative

Patricia J. Ayoubi and Alan R. Harker*

Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 74078

Received 26 May 1998/Accepted 18 August 1998

The rate, progress, and limits of trichloroethylene (TCE) degradation by Ralstonia eutropha AEK301/pYK3021 whole cells were examined in the absence of aromatic induction. At TCE concentrations up to 800 µM, degradation rates were sustained until TCE was no longer detectable. The Ks and Vmax for TCE degradation by AEK301/pYK3021 whole cells were determined to be 630 µM and 22.6 nmol/min/mg of total protein, respectively. The sustained linear rates of TCE degradation by AEK301/pYK3021 up to a concentration of 800 µM TCE suggest that solvent effects are limited during the degradation of TCE and that this construct is little affected by the formation of toxic intermediates at the TCE levels and assay duration tested. TCE degradation by this strain is subject to carbon catabolite repression.

* Corresponding author. Present address: Department of Microbiology, Brigham Young University, Provo, UT 84602-5108. Phone: (801) 378-3582. Fax: (801) 378-9197. E-mail: alharker{at}acd1.byu.edu.

Applied and Environmental Microbiology, November 1998, p. 4353-4356, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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