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Applied and Environmental Microbiology, November 1998, p. 4573-4580, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Antagonistic Activity against Helicobacter Infection In Vitro and In Vivo by the Human Lactobacillus acidophilus Strain LB

Marie-Helene Coconnier,1 Vanessa Lievin,1 Elisabeth Hemery,2 and Alain L. Servin1,*

Institut National de la Santé et de la Recherche Médicale, CJF 94.07, UFR de Pharmacie, Université Paris XI, F-92296 Châtenay-Malabry,1 and Anatomie Pathologie A, Centre Hospitalier Universitaire de Nantes, F-44000 Nantes,2 France

Received 17 March 1998/Accepted 12 August 1998

The purpose of the present study was to examine the activity of the human Lactobacillus acidophilus strain LB, which secretes an antibacterial substance(s) against Helicobacter pylori in vitro and in vivo. The spent culture supernatant (SCS) of the strain LB (LB-SCS) dramatically decreased the viability of H. pylori in vitro independent of pH and lactic acid levels. Adhesion of H. pylori to the cultured human mucosecreting HT29-MTX cells decreased in parallel with the viability of H. pylori. In conventional mice, oral treatment with the LB-SCS protected against infection with Helicobacter felis. Indeed, at both 8 and 49 days post-LB-SCS treatment (29 and 70 days postinfection), inhibition of stomach colonization by H. felis was observed, and no evidence of gastric histopathological lesions was found. LB-SCS treatment inhibits the H. pylori urease activity in vitro and in H. pylori that remained associated with the cultured human mucosecreting HT29-MTX cells. Moreover, a decrease in urease activity was detected in the stomach of the mice infected with H. felis and treated with LB-SCS.

* Corresponding author. Mailing address: UPS Faculté de Pharmacie Paris XI, CJF 94.07, INSERM, F-92296 Châtenay-Malabry, France. Phone and fax: 33.1.46.83.56.61. E-mail: alain.servin{at}cep.u-psud.fr.

Applied and Environmental Microbiology, November 1998, p. 4573-4580, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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