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Applied and Environmental Microbiology, November 1998, p. 4600-4602, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Novel Screening Method for Isolating Exopolysaccharide-Deficient Mutants

Minghsun Liu,dagger Juan E. González,Dagger Laura B. Willis, and Graham C. Walker*

Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Received 3 February 1998/Accepted 11 August 1998

A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by using this method, which might also prove useful for isolating exopolysaccharide-defective derivatives of other bacteria.

* Corresponding author. Mailing address: Department of Biology, Massachusetts Institute of Technology 68-633, 77 Massachusetts Ave., Cambridge, MA 02139. Phone: (617) 253-6716. Fax: (617) 253-2643. E-mail: gwalker{at}mit.edu.

dagger Present address: Medical Scientist Training Program and Department of Microbiology and Immunology, UCLA School of Medicine, Los Angeles, CA 90095.

Dagger Present address: Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX 75083-0688.

Applied and Environmental Microbiology, November 1998, p. 4600-4602, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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