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Applied and Environmental Microbiology, November 1998, p. 4623-4626, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Response of Salmonella choleraesuis LT2 Spheroplasts and Permeabilized Cells to the Bacteriocin AS-48

H. Abriouel,1 E. Valdivia,1 A. Gálvez,2 and M. Maqueda3,*

Dpto. Microbiología, Facultad de Ciencias, Universidad de Granada, Fuentenueva s/n,1 and Instituto de Biotecnología, Universidad de Granada,3 E-18071-Granada, and Dpto. Microbiología, Facultad de Ciencias Experimentales, Universidad de Jaén, E-23071 Jaén,2 Spain

Received 17 April 1998/Accepted 11 August 1998

The bacteriocin AS-48 was not active against intact cells of Salmonella choleraesuis LT2 at neutral pH, but it was very effective on spheroplasts, suggesting that the outer membrane (OM) acts as a protective barrier. Cells sublethally injured by heat or treated with OM-permeabilizing agents (i.e., EDTA and Tris) became sensitive to AS-48. The combination of two or more treatments decreased the amount of AS-48 required for cell killing. The activity of AS-48 against heat-injured cells did not change significantly in the pH range of 4.0 to 8.0. AS-48 showed bactericidal activity against intact cells of Salmonella at pH 4.0. The potency of AS-48 increased greatly when the bacteriocin was dissolved at pH 9.0.

* Corresponding author. Mailing address: Dpto. Microbiología (Facultad de Ciencias), Univ. Granada, Fuentenueva s/n, E-18071-Granada, Spain. Phone: (34) 58 242857. Fax: (34) 58 243246. E-mail: mmaqueda{at}goliat.ugr.es.

Applied and Environmental Microbiology, November 1998, p. 4623-4626, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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