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Applied and Environmental Microbiology, December 1998, p. 4637-4642, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Degradation of Polychlorinated Biphenyl Metabolites by Naphthalene-Catabolizing Enzymes

Diane Barriault,1 Jacinthe Durand,1 Halim Maaroufi,2 Lindsay D. Eltis,2 and Michel Sylvestre1,*

INRS-Santé, Université du Québec, Pointe-Claire, Québec, Canada H9R 1G6,1 and Départment de Biochimie, Université Laval, Ste-Foy, Québec, Canada G1K 7P42

Received 17 July 1998/Accepted 18 September 1998

The ability of the dehydrogenase and ring cleavage dioxygenase of the naphthalene degradation pathway to transform 3,4-dihydroxylated biphenyl metabolites was investigated. 1,2-Dihydro-1,2-dihydroxynaphthalene dehydrogenase was expressed as a histidine-tagged protein. The purified enzyme transformed 2,3-dihydro-2,3-dihydroxybiphenyl, 3,4-dihydro-3,4-dihydroxybiphenyl, and 3,4-dihydro-3,4-dihydroxy-2,2',5,5'-tetrachlorobiphenyl to 2,3-dihydroxybiphenyl, 3,4-dihydroxybiphenyl (3,4-DHB), and 3,4-dihydroxy-2,2',5,5'-tetrachlorobiphenyl (3,4-DH-2,2',5,5'-TCB), respectively. Our data also suggested that purified 1,2-dihydroxynaphthalene dioxygenase catalyzed the meta cleavage of 3,4-DHB in both the 2,3 and 4,5 positions. This enzyme cleaved 3,4-DH-2,2',5,5'-TCB and 3,4-DHB at similar rates. These results demonstrate the utility of the naphthalene catabolic enzymes in expanding the ability of the bph pathway to degrade polychlorinated biphenyls.

* Corresponding author. Mailing address: INRS-Santé, 245 Boul. Hymus, Pointe-Claire, Québec, Canada H9R 1G6. Phone: (514) 630-8829. Fax: (514) 630-8850. E-mail: Michel.Sylvestre{at}inrs-sante.uquebec.ca.

Applied and Environmental Microbiology, December 1998, p. 4637-4642, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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