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Applied and Environmental Microbiology, December 1998, p. 4689-4696, Vol. 64, No. 12
Department of Industrial Agrobiotechnology,
Agrotechnological Research Institute (ATO-DLO), NL-6700 AA
Wageningen, The Netherlands
Received 8 July 1998/Accepted 15 September 1998
Mannitol, a six-carbon sugar alcohol, is the main storage carbon in
the button mushroom, Agaricus bisporus. Given the
physiological importance of mannitol metabolism in growth, fruit body
development, and salt tolerance of A. bisporus, the enzyme
responsible for mannitol biosynthesis, NADP-dependent mannitol
dehydrogenase (MtDH) (EC 1.1.1.138), was purified to homogeneity, and
MtDH cDNA was cloned, sequenced, and characterized. To our
knowledge, this represents the first report on the isolation of a cDNA
encoding an NADP-dependent mannitol dehydrogenase. The
MtDH cDNA contains an open reading frame of 789 bp encoding
a protein of approximately 28 kDa. The N-terminal and internal amino
acid sequences of the deduced protein exactly matched the ones
determined from the purified MtDH subunit, whereas the amino acid
composition of the deduced protein was nearly identical to that of the
purified MtDH. The MtDH cDNA showed high homology with a
plant-induced short-chain dehydrogenase from Uromyces
fabae. Phylogenetic analysis based on amino acid
sequences from mannitol(-1-phosphate) dehydrogenases indicated a close
relationship between the substrate specificity of the enzymes and
phylogenetic differentiation. Salt-stressed fruit bodies showed an
overall increase in mannitol biosynthesis, as was evident from the
increase in MtDH activity, MtDH abundance, and MtDH RNA
accumulation. Furthermore, the MtDH transcript level seems
to be under developmental control, as MtDH RNA accumulated
during maturation of the fruit body.
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Cloning and Characterization of NADP-Mannitol Dehydrogenase cDNA
from the Button Mushroom, Agaricus bisporus, and Its
Expression in Response to NaCl Stress
*
Corresponding author. Mailing address:
Agrotechnological Research Institute (ATO-DLO), Bornsesteeg 59, P.O.
Box 17, NL-6700 AA Wageningen, The Netherlands. Phone: (31) 317 47 53 16. Fax: (31) 317 47 53 47. E-mail:
a.mooibroek{at}ato.dlo.nl.
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