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Applied and Environmental Microbiology, December 1998, p. 5000-5003, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Utility of Green Fluorescent Nucleic Acid Dyes and Aluminum Oxide Membrane Filters for Rapid Epifluorescence Enumeration of Soil and Sediment Bacteria

Markus G. Weinbauer,* Christiane Beckmann, and Manfred G. Höfle

GBF---National Research Center of Biotechnology, AG Microbial Ecology, D-38124 Braunschweig, Germany

Received 8 July 1998/Accepted 16 September 1998

High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4',6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circumvented by using green fluorescent high-affinity nucleic acid dyes and aluminum oxide membrane filters. Due to the bright staining of cells, we recommend SYBR Green II as dye.

* Corresponding author. Mailing address: GBF---National Research Center of Biotechnology, AG Microbial Ecology, Mascheroder Weg 1, D-38124 Braunschweig, Germany. Phone: 49-531-6181-440. Fax: 49-531-6181-411. E-mail: mgw{at}gbf.de.

Applied and Environmental Microbiology, December 1998, p. 5000-5003, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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