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Applied and Environmental Microbiology, December 1998, p. 5046-5048, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Changes in Bacterial Species Composition in Enrichment Cultures with Various Dilutions of Inoculum as Monitored by Denaturing Gradient Gel Electrophoresis

Colin R. Jackson,* Eric E. Roden, and Perry F. Churchill

Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama 35487

Received 15 July 1998/Accepted 8 September 1998

Denaturing gradient gel electrophoresis revealed changes in the bacterial species obtained from enrichment cultures with different inoculum dilutions. This inoculum dilution enrichment approach may facilitate the detection and isolation of a greater number of bacterial species than traditional enrichment techniques.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35487-0206. Phone: (205) 348-4883. Fax: (205) 348-1403. E-mail: cjackson{at}biology.as.ua.edu.

Applied and Environmental Microbiology, December 1998, p. 5046-5048, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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