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Applied and Environmental Microbiology, December 1998, p. 5049-5052, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Induction of the tod Operon by Trichloroethylene in Pseudomonas putida TVA8

Justin T. Shingleton,1,2 Bruce M. Applegate,1 Aaron C. Nagel,1 Paul R. Bienkowski,1,2 and Gary S. Sayler1,*

Center for Environmental Biotechnology1 and Department of Chemical Engineering,2 The University of Tennessee, Knoxville, Tennessee 37996

Received 17 July 1998/Accepted 28 September 1998

Bioluminescence, mRNA levels, and toluene degradation rates in Pseudomonas putida TVA8 were measured as a function of various concentrations of toluene and trichloroethylene (TCE). TVA8 showed an increasing bioluminescence response to increasing TCE and toluene concentrations. Compared to uninduced TVA8 cultures, todC1 mRNA levels increased 11-fold for TCE-treated cultures and 13-fold for toluene-treated cultures. Compared to uninduced P. putida F1 cultures, todC1 mRNA levels increased 4.4-fold for TCE-induced cultures and 4.9-fold for toluene-induced cultures. Initial toluene degradation rates were linearly correlated with specific bioluminescence in TVA8 cultures.

* Corresponding author. Mailing address: University of Tennessee, Center for Environmental Biotechnology, 676 Dabney Hall, Knoxville, TN 37996-1605. Phone: (423) 974-8080. Fax: (423) 974-8086. E-mail: sayler{at}utk.edu.

Applied and Environmental Microbiology, December 1998, p. 5049-5052, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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