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Applied and Environmental Microbiology, December 1998, p. 5064-5066, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Quantitative Measure of Small-Subunit rRNA Gene Sequences of the Kingdom Korarchaeota

Clifford F. Brunk1,2,* and Nicole Eis1

Lehrstuhl für Mikrobiologie und Archaeenzentrum der Universität Regensburg, Regensburg, Germany,1 and Biology Department and Molecular Biology Institute, University of California, Los Angeles, California2

Received 22 June 1998/Accepted 22 September 1998

Comparative PCR amplification of small-subunit (SSU) rRNA gene (rDNA) sequences indicates substantial preferential PCR amplification of pJP27 sequences with korarchaeote-specific PCR primers. The coamplification of a modified SSU rDNA sequence can be used as an internal standard to determine the amount of a specific SSU rDNA sequence.

* Corresponding author. Mailing address: Biology Department, University of California, Los Angeles, CA 90095-1606. Phone: (310) 825-3114. Fax: (310) 206-3987. E-mail: cbrunk{at}ucla.edu.

Applied and Environmental Microbiology, December 1998, p. 5064-5066, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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