This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suzuki, K.
Right arrow Articles by Ohmiya, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suzuki, K.
Right arrow Articles by Ohmiya, K.
Agricola
Right arrow Articles by Suzuki, K.
Right arrow Articles by Ohmiya, K.

 Previous Article  |  Next Article 

Appl Environ Microbiol, February 1998, p. 411-418, Vol. 64, No. 2
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Expression and Regulation of the Arsenic Resistance Operon of Acidiphilium multivorum AIU 301 Plasmid pKW301 in Escherichia coli

Katsuhisa Suzuki,1 Norio Wakao,2 Tetsuya Kimura,1 Kazuo Sakka,1 and Kunio Ohmiya1,*

Laboratory of Applied Microbiology, School of Bioresources, Mie University, Tsu 514,1 and Laboratory of Applied Microbiology, Faculty of Agriculture, Iwate University, Morioka 020,2 Japan

Received 16 June 1997/Accepted 9 November 1997

The arsenic resistance (ars) operon from plasmid pKW301 of Acidiphilium multivorum AIU 301 was cloned and sequenced. This DNA sequence contains five genes in the following order: arsR, arsD, arsA, arsB, arsC. The predicted amino acid sequences of all of the gene products are homologous to the amino acid sequences of the ars gene products of Escherichia coli plasmid R773 and IncN plasmid R46. The ars operon cloned from A. multivorum conferred resistance to arsenate and arsenite on E. coli. Expression of the ars genes with the bacteriophage T7 RNA polymerase-promoter system allowed E. coli to overexpress ArsD, ArsA, and ArsC but not ArsR or ArsB. The apparent molecular weights of ArsD, ArsA, and ArsC were 13,000, 64,000, and 16,000, respectively. A primer extension analysis showed that the ars mRNA started at a position 19 nucleotides upstream from the arsR ATG in E. coli. Although the arsR gene of A. multivorum AIU 301 encodes a polypeptide of 84 amino acids that is smaller and less homologous than any of the other ArsR proteins, inactivation of the arsR gene resulted in constitutive expression of the ars genes, suggesting that ArsR of pKW301 controls the expression of this operon.

* Corresponding author. Mailing address: Laboratory of Applied Microbiology, School of Bioresources, Mie University, 1515 Kamihama-cho, Tsu 514, Japan. Phone and fax: 81-592-9622/9634. E-mail: ohmiya{at}bio.mie-u.ac.jp.




This article has been cited by other articles:

  • Ordonez, E., Letek, M., Valbuena, N., Gil, J. A., Mateos, L. M. (2005). Analysis of Genes Involved in Arsenic Resistance in Corynebacterium glutamicum ATCC 13032. Appl. Environ. Microbiol. 71: 6206-6215 [Abstract] [Full Text]  
  • Li, R., Haile, J. D., Kennelly, P. J. (2003). An Arsenate Reductase from Synechocystis sp. Strain PCC 6803 Exhibits a Novel Combination of Catalytic Characteristics. J. Bacteriol. 185: 6780-6789 [Abstract] [Full Text]  
  • Dopson, M., Baker-Austin, C., Koppineedi, P. R., Bond, P. L. (2003). Growth in sulfidic mineral environments: metal resistance mechanisms in acidophilic micro-organisms. Microbiology 149: 1959-1970 [Abstract] [Full Text]  
  • Saltikov, C. W., Olson, B. H. (2002). Homology of Escherichia coli R773 arsA, arsB, and arsC Genes in Arsenic-Resistant Bacteria Isolated from Raw Sewage and Arsenic-Enriched Creek Waters. Appl. Environ. Microbiol. 68: 280-288 [Abstract] [Full Text]  
  • Butcher, B. G., Deane, S. M., Rawlings, D. E. (2000). The Chromosomal Arsenic Resistance Genes of Thiobacillus ferrooxidans Have an Unusual Arrangement and Confer Increased Arsenic and Antimony Resistance to Escherichia coli. Appl. Environ. Microbiol. 66: 1826-1833 [Abstract] [Full Text]  
  • Loh, J., Stacey, M. G., Sadowsky, M. J., Stacey, G. (1999). The Bradyrhizobium japonicum nolA Gene Encodes Three Functionally Distinct Proteins. J. Bacteriol. 181: 1544-1554 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»