This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Metcalf, W. W.
Right arrow Articles by Wolfe, R. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Metcalf, W. W.
Right arrow Articles by Wolfe, R. S.
Agricola
Right arrow Articles by Metcalf, W. W.
Right arrow Articles by Wolfe, R. S.

 Previous Article  |  Next Article 

Appl Environ Microbiol, February 1998, p. 768-770, Vol. 64, No. 2
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

An Anaerobic, Intrachamber Incubator for Growth of Methanosarcina spp. on Methanol-Containing Solid Media

William W. Metcalf,* Jun Kai Zhang, and Ralph S. Wolfe

Department of Microbiology, University of Illinois, Urbana, Illinois 61801

Received 22 September 1997/Accepted 1 December 1997

To simplify the incubation of Methanosarcina spp. on solid agar medium, a two-port, manual, rectangular air lock was modified to serve as an anaerobic incubator. In one operation, it is possible to incubate 153 petri plates, the equivalent of 11 standard anaerobic jars, with plating efficiencies identical to those of traditional protocols.

* Corresponding author. Mailing address: Department of Microbiology, B103 Chemical and Life Sciences Laboratory, University of Illinois, 601 South Goodwin Ave., Urbana, IL 61801. Phone: (217) 244-1943. Fax: (217) 244-6697. E-mail: metcalf{at}uiuc.edu.




This article has been cited by other articles:

  • Opulencia, R. B., Bose, A., Metcalf, W. W. (2009). Physiology and Posttranscriptional Regulation of Methanol:Coenzyme M Methyltransferase Isozymes in Methanosarcina acetivorans C2A. J. Bacteriol. 191: 6928-6935 [Abstract] [Full Text]  
  • Bose, A., Pritchett, M. A., Metcalf, W. W. (2008). Genetic Analysis of the Methanol- and Methylamine-Specific Methyltransferase 2 Genes of Methanosarcina acetivorans C2A. J. Bacteriol. 190: 4017-4026 [Abstract] [Full Text]  
  • Welander, P. V., Metcalf, W. W. (2008). Mutagenesis of the C1 Oxidation Pathway in Methanosarcina barkeri: New Insights into the Mtr/Mer Bypass Pathway. J. Bacteriol. 190: 1928-1936 [Abstract] [Full Text]  
  • Bose, A., Pritchett, M. A., Rother, M., Metcalf, W. W. (2006). Differential Regulation of the Three Methanol Methyltransferase Isozymes in Methanosarcina acetivorans C2A.. J. Bacteriol. 188: 7274-7283 [Abstract] [Full Text]  
  • Pritchett, M. A., Zhang, J. K., Metcalf, W. W. (2004). Development of a Markerless Genetic Exchange Method for Methanosarcina acetivorans C2A and Its Use in Construction of New Genetic Tools for Methanogenic Archaea. Appl. Environ. Microbiol. 70: 1425-1433 [Abstract] [Full Text]  
  • Galagan, J. E., Nusbaum, C., Roy, A., Endrizzi, M. G., Macdonald, P., FitzHugh, W., Calvo, S., Engels, R., Smirnov, S., Atnoor, D., Brown, A., Allen, N., Naylor, J., Stange-Thomann, N., DeArellano, K., Johnson, R., Linton, L., McEwan, P., McKernan, K., Talamas, J., Tirrell, A., Ye, W., Zimmer, A., Barber, R. D., Cann, I., Graham, D. E., Grahame, D. A., Guss, A. M., Hedderich, R., Ingram-Smith, C., Kuettner, H. C., Krzycki, J. A., Leigh, J. A., Li, W., Liu, J., Mukhopadhyay, B., Reeve, J. N., Smith, K., Springer, T. A., Umayam, L. A., White, O., White, R. H., de Macario, E. C., Ferry, J. G., Jarrell, K. F., Jing, H., Macario, A. J.L., Paulsen, I., Pritchett, M., Sowers, K. R., Swanson, R. V., Zinder, S. H., Lander, E., Metcalf, W. W., Birren, B. (2002). The Genome of M. acetivorans Reveals Extensive Metabolic and Physiological Diversity. Genome Res 12: 532-542 [Abstract] [Full Text]  
  • Zhang, J. K., Pritchett, M. A., Lampe, D. J., Robertson, H. M., Metcalf, W. W. (2000). In vivo transposon mutagenesis of the methanogenic archaeon Methanosarcina acetivorans C2A using a modified version of the insect mariner-family transposable element Himar1. Proc. Natl. Acad. Sci. USA 10.1073/pnas.160272597v1 [Abstract] [Full Text]  
  • Boccazzi, P., Zhang, J. K., Metcalf, W. W. (2000). Generation of Dominant Selectable Markers for Resistance to Pseudomonic Acid by Cloning and Mutagenesis of the ileS Gene from the Archaeon Methanosarcina barkeri Fusaro. J. Bacteriol. 182: 2611-2618 [Abstract] [Full Text]  
  • Macario, A. J. L., Lange, M., Ahring, B. K., De Macario, E. C. (1999). Stress Genes and Proteins in the Archaea. Microbiol. Mol. Biol. Rev. 63: 923-967 [Abstract] [Full Text]  
  • Wolfe, R. S. (1999). Anaerobic Life---a Centennial View. J. Bacteriol. 181: 3317-3320 [Full Text]  
  • Zhang, J. K., Pritchett, M. A., Lampe, D. J., Robertson, H. M., Metcalf, W. W. (2000). In vivo transposon mutagenesis of the methanogenic archaeon Methanosarcina acetivorans C2A using a modified version of the insect mariner-family transposable element Himar1. Proc. Natl. Acad. Sci. USA 97: 9665-9670 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»