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Appl Environ Microbiol, February 1998, p. 768-770, Vol. 64, No. 2
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

An Anaerobic, Intrachamber Incubator for Growth of Methanosarcina spp. on Methanol-Containing Solid Media

William W. Metcalf,* Jun Kai Zhang, and Ralph S. Wolfe

Department of Microbiology, University of Illinois, Urbana, Illinois 61801

Received 22 September 1997/Accepted 1 December 1997

To simplify the incubation of Methanosarcina spp. on solid agar medium, a two-port, manual, rectangular air lock was modified to serve as an anaerobic incubator. In one operation, it is possible to incubate 153 petri plates, the equivalent of 11 standard anaerobic jars, with plating efficiencies identical to those of traditional protocols.

* Corresponding author. Mailing address: Department of Microbiology, B103 Chemical and Life Sciences Laboratory, University of Illinois, 601 South Goodwin Ave., Urbana, IL 61801. Phone: (217) 244-1943. Fax: (217) 244-6697. E-mail: metcalf{at}uiuc.edu.




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