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Appl Environ Microbiol, February 1998, p. 779-783, Vol. 64, No. 2
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The H+-ATPase in the Plasma Membrane of Saccharomyces cerevisiae Is Activated during Growth Latency in Octanoic Acid-Supplemented Medium Accompanying the Decrease in Intracellular pH and Cell Viability

Cristina A. Viegas, Paulo F. Almeida, Miguel Cavaco, and Isabel Sá-Correia*

Centro de Engenharia Biológica e Química, Secção de Biotecnologia, Instituto Superior Técnico, Lisbon, Portugal

Received 7 August 1997/Accepted 22 November 1997

Saccharomyces cerevisiae plasma membrane H+-ATPase activity was stimulated during octanoic acid-induced latency, reaching maximal values at the early stages of exponential growth. The time-dependent pattern of ATPase activation correlated with the decrease of cytosolic pH (pHi). The cell population used as inoculum exhibited a significant heterogeneity of pHi, and the fall of pHi correlated with the loss of cell viability as determined by plate counts. When exponential growth started, only a fraction of the initial population was still viable, consistent with the role of the physiology and number of viable cells in the inoculum in the duration of latency under acid stress.

* Corresponding author. Mailing address: Centro de Engenharia Biológica e Química, Secção de Biotecnologia, Instituto Superior Técnico, Av. Rovisco Pais, 1096 Lisbon Codex, Portugal. Phone: 351-1-8417233. Fax: 351-1-8480072. E-mail: pcisc{at}alfa.ist.utl.pt.




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