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Appl Environ Microbiol, February 1998, p. 779-783, Vol. 64, No. 2
Centro de Engenharia Biológica e
Química, Secção de Biotecnologia, Instituto Superior
Técnico, Lisbon, Portugal
Received 7 August 1997/Accepted 22 November 1997
Saccharomyces cerevisiae plasma membrane
H+-ATPase activity was stimulated during octanoic
acid-induced latency, reaching maximal values at the early stages of
exponential growth. The time-dependent pattern of ATPase activation
correlated with the decrease of cytosolic pH (pHi). The
cell population used as inoculum exhibited a significant heterogeneity
of pHi, and the fall of pHi correlated with the loss of cell viability as determined by plate counts. When exponential growth started, only a fraction of the initial population was still
viable, consistent with the role of the physiology and number of viable
cells in the inoculum in the duration of latency under acid stress.
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
The H+-ATPase in the Plasma Membrane of
Saccharomyces cerevisiae Is Activated during Growth Latency
in Octanoic Acid-Supplemented Medium Accompanying the Decrease in
Intracellular pH and Cell Viability
*
Corresponding author. Mailing address: Centro de
Engenharia Biológica e Química, Secção de
Biotecnologia, Instituto Superior Técnico, Av. Rovisco Pais, 1096 Lisbon Codex, Portugal. Phone: 351-1-8417233. Fax: 351-1-8480072. E-mail: pcisc{at}alfa.ist.utl.pt.
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