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Appl Environ Microbiol, February 1998, p. 793-794, Vol. 64, No. 2
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Construction of a Sorbitol-Based Vector for Expression of Heterologous Proteins in Saccharomyces cerevisiae

T. McGonigal, P. Bodelle, C. Schopp, and A. V. Sarthy^*

Infectious Disease Research, Pharmaceutical Products Division, Abbott Laboratories, Abbott Park, Illinois 60064-3500

Received 23 June 1997/Accepted 25 November 1997

A new inducible yeast expression vector, pXS7, was constructed by using the promoter and terminator sequences from the Saccharomyces cerevisiae SOR1 gene, which codes for the sorbitol dehydrogenase protein. We cloned the coding sequence of the Saccharomyces YEF3 gene in this vector and demonstrated an increase in YEF3 protein levels when cells were grown in the presence of the sugar sorbitol.

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^* Corresponding author. Mailing address: Abbott Laboratories, Department 47M, Building AP9A, 100 Abbott Park Rd., Abbott Park, IL 60064-3500. Phone: (847) 937-1571. Fax: (847) 938-1021. E-mail: aparna.sarthy{at}abbott.com.

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