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 Previous Article

Appl Environ Microbiol, February 1998, p. 800-803, Vol. 64, No. 2
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Purification and Characterization of Anti-Listeria Compounds Produced by Geotrichum candidum

V. Dieuleveux,1 D. Van Der Pyl,2 J. Chataud,3 and M. Gueguen1,*

Laboratoire de Microbiologie Alimentaire, Institut de Recherche en Biologie Appliquée, Université de Caen, 14032 Caen,1 Centre de Recherche de Vitry-Alfortville, Rhône-Poulenc Rorer, 94403 Vitry-sur-Seine,2 and Laboratoire de Recherche-Développement, TEXEL, 86220 Dangé-Saint-Romain,3 France

Received 18 March 1997/Accepted 21 November 1997

Geotrichum candidum can produce and excrete compounds that inhibit Listeria monocytogenes. These were purified by ultrafiltration, centrifugal partition chromatography, thin-layer chromatography, gel filtration, and high-pressure liquid chromatography, and analyzed by liquid chromatography-mass spectrometry, infrared spectrometry, nuclear magnetic resonance spectrometry, and optical rotation. Two inhibitors were identified: D-3-phenyllactic acid and D-3-indollactic acid.

* Corresponding author. Mailing address: Laboratoire de Microbiologie Alimentaire, IBBA, Université de Caen, 14032 Caen cedex, France. Phone: 02. 31. 56. 56. 21. Fax: 02. 31. 56. 61. 79. E-mail: gueguen{at}ibba.unicaen.Fr.






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Copyright © 1998 by the American Society for Microbiology. All rights reserved.