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Appl Environ Microbiol, March 1998, p. 1157-1160, Vol. 64, No. 3
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Direct Detection of Viable Bacteria, Molds, and Yeasts by Reverse Transcriptase PCR in Contaminated Milk Samples after Heat Treatment

Marc Vaitilingom, Francois Gendre, and Pierre Brignon*

Tepral, Beverage Division Research Center of Danone Group, 67084 Strasbourg Cedex, France

Received 14 July 1997/Accepted 29 December 1997

A fast, sensitive, and target contaminant-modulable method was developed to detect viable bacteria, molds, and yeasts after heat treatment. By reverse transcriptase PCR with elongation factor gene (EF-Tu or EF-1alpha )-specific primers, the detection level was 10 cells ml of milk-1. The simplicity and rapidity (4 h) of the procedure suggests that this method may be easily transposable to other foods and other contaminants.

* Corresponding author. Mailing address: Tepral, 68, Route d'Oberhausbergen, 67037 Strasbourg Cedex, France. Phone: (333)88.27.40.98. Fax: (333)88.27.40.53. E-mail: pbrignon{at}www.tepral.fr.




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