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Appl Environ Microbiol, April 1998, p. 1230-1236, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Specificity of Milk Peptide Utilization by Lactococcus lactis

Vincent Juillard,1,* Alain Guillot,2 Dominique Le Bars,2 and Jean-Claude Gripon2

Unité de Recherches Laitières et Génétique Appliquée1 and Unité de Biochimie et Structure des Protéines,2 Institut National de la Recherche Agronomique, Centre de Recherches de Jouy-en-Josas, 78350 Jouy-en-Josas, France

Received 16 September 1997/Accepted 29 January 1998

To study the substrate specificity of the oligopeptide transport system of Lactococcus lactis for its natural substrates, the growth of L. lactis MG1363 was studied in a chemically defined medium containing milk peptides or a tryptic digest of alpha s2-casein as the source of amino acids. Peptides were separated into acidic, neutral, and basic pools by solid-phase extraction or by cation-exchange liquid chromatography. Their ability to sustain growth and the time course of their utilization demonstrated the preferential use of hydrophobic basic peptides with molecular masses ranging between 600 and 1,100 Da by L. lactis MG1363 and the inability to use large, acidic peptides. These peptide utilization preferences reflect the substrate specificity of the oligopeptide transport system of the strain, since no significant cell lysis was inferred. Considering the free amino acid content of milk and these findings on peptide utilization, it was demonstrated that the cessation of growth of L. lactis MG1363 in milk was due to deprivation of leucine and methionine.

* Corresponding author. Mailing address: Unité de Recherches Laitières et Génétique Appliquée, Institut National de la Recherche Agronomique, Centre de Recherches de Jouy-en-Josas, 78350 Jouy-en-Josas, France. Phone: (33) 134 652 068. Fax: (33) 134 652 065. E-mail: juillard{at}jouy.inra.fr.




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