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Appl Environ Microbiol, April 1998, p. 1242-1245, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

PCR for Detection of Shigella spp. in Mayonnaise

Eduardo Villalobo* and Antonio Torres

Departamento Microbiología, Facultad Biología, Universidad Sevilla, 41080 Seville, Spain

Received 1 August 1997/Accepted 31 January 1998

The use of PCR to amplify a specific virA gene fragment serves as a highly specific and sensitive method to detect virulent bacteria of the genus Shigella and enteroinvasive Escherichia coli. Amplification of a 215-bp DNA band was obtained by using isolated genomic DNA of Shigella, individual cells of Shigella dysenteriae, and mayonnaise contaminated with S. dysenteriae. Moreover, a multiplex PCR with specific (virA) and bacterium-restricted (16S ribosomal DNA) primers generated an amplification product of approximately 755 bp for all bacteria tested and an additional 215-bp product for Shigella and enteroinvasive E. coli.

* Corresponding author. Mailing address: Dpto. Microbiología, Fac. Biología, Univ. Sevilla Apdo. 1095, 41080 Seville, Spain. Phone: 34 5 455 71 15. Fax: 34 5 455 78 30. E-mail: evpolo{at}cica.es.




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Copyright © 1998 by the American Society for Microbiology. All rights reserved.