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Appl Environ Microbiol, April 1998, p. 1270-1275, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Reductive Dechlorination of Tetrachloroethene to Ethene by a Two-Component Enzyme Pathway

Jon K. Magnuson,1,* Robert V. Stern,1,dagger James M. Gossett,2 Stephen H. Zinder,3 and David R. Burris1

USAF Armstrong Laboratory, Environics Directorate, Tyndall Air Force Base, Florida 32403-5323,1 and School of Civil and Environmental Engineering,2 and Section of Microbiology,3 Cornell University, Ithaca, New York 14853

Received 29 October 1997/Accepted 21 January 1998

Two membrane-bound, reductive dehalogenases that constitute a novel pathway for complete dechlorination of tetrachloroethene (perchloroethylene [PCE]) to ethene were partially purified from an anaerobic microbial enrichment culture containing Dehalococcoides ethenogenes 195. When titanium(III) citrate and methyl viologen were used as reductants, PCE-reductive dehalogenase (PCE-RDase) (51 kDa) dechlorinated PCE to trichloroethene (TCE) at a rate of 20 µmol/min/mg of protein. TCE-reductive dehalogenase (TCE-RDase) (61 kDa) dechlorinated TCE to ethene. TCE, cis-1,2-dichloroethene, and 1,1-dichloroethene were dechlorinated at similar rates, 8 to 12 µmol/min/mg of protein. Vinyl chloride and trans-1,2-dichloroethene were degraded at rates which were approximately 2 orders of magnitude lower. The light-reversible inhibition of TCE-RDase by iodopropane and the light-reversible inhibition of PCE-RDase by iodoethane suggest that both of these dehalogenases contain Co(I) corrinoid cofactors. Isolation and characterization of these novel bacterial enzymes provided further insight into the catalytic mechanisms of biological reductive dehalogenation.

* Corresponding author. Present address: U. S. Army ERDEC, SCBRD-RTL, Bldg. E3150, Aberdeen Proving Ground, MD 21010. Phone: (410) 612-7831. Fax: (410) 671-2081. E-mail: jkmagnus{at}c-mail.apgea.army.mil.

dagger Present address: Department of Biological Sciences, Southeastern Louisiana University, Hammond, LA 70402.




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