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Appl Environ Microbiol, April 1998, p. 1270-1275, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Reductive Dechlorination of Tetrachloroethene to
Ethene by a Two-Component Enzyme Pathway
Jon K.
Magnuson,1,*
Robert V.
Stern,1,
James M.
Gossett,2
Stephen H.
Zinder,3 and
David R.
Burris1
USAF Armstrong Laboratory, Environics
Directorate, Tyndall Air Force Base, Florida
32403-5323,1 and
School of Civil and
Environmental Engineering,2 and
Section
of Microbiology,3 Cornell University,
Ithaca, New York 14853
Received 29 October 1997/Accepted 21 January 1998
Two membrane-bound, reductive dehalogenases that constitute a novel
pathway for complete dechlorination of tetrachloroethene (perchloroethylene [PCE]) to ethene were partially purified from an
anaerobic microbial enrichment culture containing Dehalococcoides ethenogenes 195. When titanium(III) citrate and methyl viologen were used as reductants, PCE-reductive dehalogenase (PCE-RDase) (51 kDa) dechlorinated PCE to trichloroethene (TCE) at a rate of 20 µmol/min/mg of protein. TCE-reductive dehalogenase (TCE-RDase) (61 kDa) dechlorinated TCE to ethene. TCE,
cis-1,2-dichloroethene, and 1,1-dichloroethene were
dechlorinated at similar rates, 8 to 12 µmol/min/mg of protein. Vinyl
chloride and trans-1,2-dichloroethene were degraded at
rates which were approximately 2 orders of magnitude lower. The
light-reversible inhibition of TCE-RDase by iodopropane and
the light-reversible inhibition of PCE-RDase by iodoethane suggest that
both of these dehalogenases contain Co(I) corrinoid cofactors.
Isolation and characterization of these novel bacterial enzymes
provided further insight into the catalytic mechanisms of
biological reductive dehalogenation.
*
Corresponding author. Present address: U. S.
Army ERDEC, SCBRD-RTL, Bldg. E3150, Aberdeen Proving Ground, MD
21010. Phone: (410) 612-7831. Fax: (410) 671-2081. E-mail:
jkmagnus{at}c-mail.apgea.army.mil.

Present address: Department of Biological Sciences, Southeastern
Louisiana University, Hammond, LA 70402.
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