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Appl Environ Microbiol, April 1998, p. 1442-1446, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Analysis of the -1,3-Glucanolytic System of the Biocontrol Agent Trichoderma harzianum

Soledad Vázquez-Garcidueñas,^1,2 Carlos A. Leal-Morales,² and Alfredo Herrera-Estrella^1,*

Centro de Investigación y Estudios Avanzados, Unidad de Biotecnología e Ingeniería Genética de Plantas, Irapuato, Gto., 36500,¹ and Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato, Guanajuato, Gto., 36000,² Mexico

Received 6 October 1997/Accepted 25 January 1998

The biocontrol agent Trichoderma harzianum IMI206040 secretes -1,3-glucanases in the presence of different glucose polymers and fungal cell walls. The level of -1,3-glucanase activity secreted was found to be proportional to the amount of glucan present in the inducer. The fungus produces at least seven extracellular -1,3-glucanases upon induction with laminarin, a soluble -1,3-glucan. The molecular weights of five of these enzymes fall in the range from 60,000 to 80,000, and their pIs are 5.0 to 6.8. In addition, a 35-kDa protein with a pI of 5.5 and a 39-kDa protein are also secreted. Glucose appears to inhibit the formation of all of the inducible -1,3-glucanases detected. A 77-kDa glucanase was partially purified from the laminarin culture filtrate. This enzyme is glycosylated and belongs to the exo--1,3-glucanase group. The properties of this complex group of enzymes suggest that the enzymes might play different roles in host cell wall lysis during mycoparasitism.

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^* Corresponding author. Mailing address: Centro de Investigación y Estudios Avanzados, Unidad Irapuato, A.P. 629, 36500 Irapuato, Gto., Mexico. Phone: 52 462 39658. Fax: 52 462 45489. E-mail: aherrera{at}irapuato.ira.cinvestav.mx.

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