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Appl Environ Microbiol, April 1998, p. 1563-1565, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Changes in Permeability of Brush Border Membrane Vesicles from Spodoptera littoralis Midgut Induced by Insecticidal Crystal Proteins from Bacillus thuringiensis

B. Escriche,¹ N. De Decker,¹ J. Van Rie,² S. Jansens,² and E. Van Kerkhove^1,*

Department Medische Basis Wetenschappen, Limburgs Universitair Centrum, B-3590 Diepenbeek,¹ and Plant Genetic Systems, B-9000 Ghent,² Belgium

Received 15 October 1997/Accepted 13 January 1998

Bacillus thuringiensis insecticidal crystal proteins (ICPs) are thought to induce pore formation in midgut cell membranes of susceptible insects. Cry1Ca, which is significantly active in Spodoptera littoralis, made brush border membrane vesicles permeable to KCl (osmotic swelling was monitored by the light scattering technique); the marginally active ICPs Cry1Aa, Cry1Ab, and Cry1Ac did not.

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^* Corresponding author. Mailing address: Department MBW, Limburgs Universitair Centrum, B-3590 Diepenbeek, Belgium. Phone: 32 11 268533. Fax: 32 11 268199. E-mail: evankerk{at}luc.ac.be.