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Appl Environ Microbiol, April 1998, p. 1580-1583, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea

Nancy B. Fitzgerald, R. Samuel English, Jay S. Lampel, and Thomas J. Vanden Boom*

Fermentation Microbiology Research and Development, Abbott Laboratories, North Chicago, Illinois 60064

Received 29 October 1997/Accepted 11 January 1998

We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for the introduction of DNA into filamentous microorganisms otherwise recalcitrant to transformation techniques.

* Corresponding author. Mailing address: Fermentation Microbiology Research and Development, Abbott Laboratories, North Chicago, IL 60064. Phone: (847) 937-4470. Fax: (847) 938-7509. E-mail: thomas.vandenboom{at}abbott.com.




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Copyright © 1998 by the American Society for Microbiology. All rights reserved.