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Appl Environ Microbiol, April 1998, p. 1584-1586, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

DNA Sequence Similarity between California Isolates of Cryptosporidium parvum

Maria das Graças C. Pereira,¹ Edward R. Atwill,^1,* Melissa R. Crawford,² and Rance B. Lefebvre²

Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California, Davis, Tulare, California 93274,¹ and Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, California 95616²

Received 1 December 1997/Accepted 28 January 1998

We evaluated whether nucleic acid amplification with primers specific for Cryptosporidium parvum followed by automated DNA sequence analysis of the PCR amplicons could differentiate between California isolates of C. parvum obtained from livestock, humans, and feral pigs. Almost complete sequence identity existed among the livestock isolates and between the livestock and human isolates. DNA sequences from feral pig isolates differed from those from livestock and humans by 1.0 to 1.2%. The reference sequence obtained by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg. 45:688-694, 1991.) differed from California isolates of C. parvum by 1.8 to 3.2%. These data suggest that DNA sequence analysis of the amplicon of Laxer et al. does not allow for differentiation between various strains of C. parvum or that our collection of isolates obtained from various hosts from across California was limited to one strain of C. parvum.

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^* Corresponding author. Mailing address: Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, 18830 Rd. 112, University of California, Davis, Tulare, CA 93274. Phone: (209) 688-1731. Fax: (209) 686-4231. E-mail: ratwill{at}vmtrc.ucdavis.edu.

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