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Appl Environ Microbiol, May 1998, p. 1601-1606, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of Laccases and Peroxidases from Wood-Rotting Fungi (Family Coprinaceae)

Marion Heinzkill,1 Lisbeth Bech,2 Torben Halkier,2 Palle Schneider,2 and Timm Anke1,*

Department of Biotechnology, University of Kaiserslautern, D-67663 Kaiserslautern, Germany,1 and Novo Nordisk A/S, DK-2880 Bagsvaerd, Denmark2

Received 27 August 1997/Accepted 21 January 1998

Panaeolus sphinctrinus, Panaeolus papilionaceus, and Coprinus friesii are described as producers of ligninolytic enzymes. P. papilionaceus and P. sphinctrinus both produced a laccase. In addition, P. sphinctrinus produced a manganese peroxidase. C. friesii secreted a laccase and two peroxidases similar to the peroxidase of Coprinus cinereus. The purified laccases and peroxidases were characterized by broad substrate specificities, significant enzyme activities at alkaline pH values, and remarkably high pH optima. The two peroxidases of C. friesii remained active at pH 7.0 and 60°C for up to 60 min of incubation. The peroxidases were inhibited by sodium azide and ethylene glycol-bis(beta -aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), whereas the laccases were inhibited by sodium azide and N,N-diethyldithiocarbamic acid. As determined by native polyacrylamide gel electrophoresis and isoelectric focusing, all three fungi produced laccase isoenzymes.

* Corresponding author. Mailing address: Department of Biotechnology, University of Kaiserslautern, Paul-Ehrlich-Str. 23, D-67663 Kaiserslautern, Germany. Phone: (01149) 631/205-2697. Fax: (01149) 631/205-2999. E-mail: anke{at}rhrk.uni-kl.de.

Appl Environ Microbiol, May 1998, p. 1601-1606, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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