This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fulthorpe, R. R.
Right arrow Articles by Tiedje, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fulthorpe, R. R.
Right arrow Articles by Tiedje, J. M.
Agricola
Right arrow Articles by Fulthorpe, R. R.
Right arrow Articles by Tiedje, J. M.

 Previous Article  |  Next Article 

Appl Environ Microbiol, May 1998, p. 1620-1627, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

High Levels of Endemicity of 3-Chlorobenzoate-Degrading Soil Bacteria

R. R. Fulthorpe,1,* A. N. Rhodes,2 and J. M. Tiedje3

University of Toronto at Scarborough, Toronto, Ontario, Canada M1C 1A41; Department of Biology, U.S. Air Force Academy, Colorado Springs, Colorado 808402; and Center for Microbial Ecology and Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824-13253

Received 17 July 1997/Accepted 20 January 1998

Soils samples were obtained from pristine ecosystems in six regions on five continents. Two of the regions were boreal forests, and the other four were Mediterranean ecosystems. Twenty-four soil samples from each of four or five sites in each of the regions were enriched by using 3-chlorobenzoate (3CBA), and 3CBA mineralizers were isolated from most samples. These isolates were analyzed for the ability to mineralize 3CBA, and genotypes were determined with repetitive extragenic palindromic PCR genomic fingerprints and restriction digests of the 16S rRNA genes (amplified ribosomal DNA restriction analysis [ARDRA]). We found that our collection of 150 stable 3CBA-mineralizing isolates included 48 genotypes and 44 ARDRA types, which formed seven distinct clusters. The majority (91%) of the genotypes were unique to the sites from which they were isolated, and each genotype was found only in the region from which it was isolated. A total of 43 of the 44 ARDRA types were found in only one region. A few genotypes were repeatedly found in one region but not in any other continental region, suggesting that they are regionally endemic. A correlation between bacterial genotype and vegetative community was found for the South African samples. These results suggest that the ability to mineralize 3CBA is distributed among very diverse genotypes and that the genotypes are not globally dispersed.


* Corresponding author. Mailing address: Physical Sciences Division, University of Toronto at Scarborough, 1265 Military Trail, Scarborough, Ontario M1C 1A4, Canada. Phone: (416) 287-7221. Fax: (416) 287-7204. E-mail: fulthorpe{at}scar.utoronto.ca.


Appl Environ Microbiol, May 1998, p. 1620-1627, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Wawrik, B., Kutliev, D., Abdivasievna, U. A., Kukor, J. J., Zylstra, G. J., Kerkhof, L. (2007). Biogeography of Actinomycete Communities and Type II Polyketide Synthase Genes in Soils Collected in New Jersey and Central Asia. Appl. Environ. Microbiol. 73: 2982-2989 [Abstract] [Full Text]  
  • Whitaker, R. J (2006). Allopatric origins of microbial species. Phil Trans R Soc B 361: 1975-1984 [Abstract] [Full Text]  
  • Curtis, T. P, Head, I. M, Lunn, M., Woodcock, S., Schloss, P. D, Sloan, W. T (2006). What is the extent of prokaryotic diversity?. Phil Trans R Soc B 361: 2023-2037 [Abstract] [Full Text]  
  • Fierer, N., Jackson, R. B. (2006). From the Cover: The diversity and biogeography of soil bacterial communities. Proc. Natl. Acad. Sci. USA 103: 626-631 [Abstract] [Full Text]  
  • Zhou, J., Xia, B., Huang, H., Palumbo, A. V., Tiedje, J. M. (2004). Microbial Diversity and Heterogeneity in Sandy Subsurface Soils. Appl. Environ. Microbiol. 70: 1723-1734 [Abstract] [Full Text]  
  • Oda, Y., Star, B., Huisman, L. A., Gottschal, J. C., Forney, L. J. (2003). Biogeography of the Purple Nonsulfur Bacterium Rhodopseudomonas palustris. Appl. Environ. Microbiol. 69: 5186-5191 [Abstract] [Full Text]  
  • Bent, S. J., Gucker, C. L., Oda, Y., Forney, L. J. (2003). Spatial Distribution of Rhodopseudomonas palustris Ecotypes on a Local Scale. Appl. Environ. Microbiol. 69: 5192-5197 [Abstract] [Full Text]  
  • Whitaker, R. J., Grogan, D. W., Taylor, J. W. (2003). Geographic Barriers Isolate Endemic Populations of Hyperthermophilic Archaea. Science 301: 976-978 [Abstract] [Full Text]  
  • Eriksson, M., Sodersten, E., Yu, Z., Dalhammar, G., Mohn, W. W. (2003). Degradation of Polycyclic Aromatic Hydrocarbons at Low Temperature under Aerobic and Nitrate-Reducing Conditions in Enrichment Cultures from Northern Soils. Appl. Environ. Microbiol. 69: 275-284 [Abstract] [Full Text]  
  • Curtis, T. P., Sloan, W. T., Scannell, J. W. (2002). From the Cover: Estimating prokaryotic diversity and its limits. Proc. Natl. Acad. Sci. USA 99: 10494-10499 [Abstract] [Full Text]  
  • Oda, Y., Wanders, W., Huisman, L. A., Meijer, W. G., Gottschal, J. C., Forney, L. J. (2002). Genotypic and Phenotypic Diversity within Species of Purple Nonsulfur Bacteria Isolated from Aquatic Sediments. Appl. Environ. Microbiol. 68: 3467-3477 [Abstract] [Full Text]  
  • Cho, J.-C., Tiedje, J. M. (2000). Biogeography and Degree of Endemicity of Fluorescent Pseudomonas Strains in Soil. Appl. Environ. Microbiol. 66: 5448-5456 [Abstract] [Full Text]  
  • Grundmann, G. L., Normand, P. (2000). Microscale Diversity of the Genus Nitrobacter in Soil on the Basis of Analysis of Genes Encoding rRNA. Appl. Environ. Microbiol. 66: 4543-4546 [Abstract] [Full Text]  
  • Bull, A. T., Ward, A. C., Goodfellow, M. (2000). Search and Discovery Strategies for Biotechnology: the Paradigm Shift. Microbiol. Mol. Biol. Rev. 64: 573-606 [Abstract] [Full Text]  
  • Song, B., Palleroni, N. J., Häggblom, M. M. (2000). Isolation and Characterization of Diverse Halobenzoate-Degrading Denitrifying Bacteria from Soils and Sediments. Appl. Environ. Microbiol. 66: 3446-3453 [Abstract] [Full Text]  
  • Peel, M. C., Wyndham, R. C. (1999). Selection of clc, cba, and fcb Chlorobenzoate-Catabolic Genotypes from Groundwater and Surface Waters Adjacent to the Hyde Park, Niagara Falls, Chemical Landfill. Appl. Environ. Microbiol. 65: 1627-1635 [Abstract] [Full Text]  
  • Ogawa, N., Miyashita, K. (1999). The Chlorocatechol-Catabolic Transposon Tn5707 of Alcaligenes eutrophus NH9, Carrying a Gene Cluster Highly Homologous to That in the 1,2,4-Trichlorobenzene-Degrading Bacterium Pseudomonas sp. Strain P51, Confers the Ability To Grow on 3-Chlorobenzoate. Appl. Environ. Microbiol. 65: 724-731 [Abstract] [Full Text]