This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coschigano, P. W.
Right arrow Articles by Young, L. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coschigano, P. W.
Right arrow Articles by Young, L. Y.
Agricola
Right arrow Articles by Coschigano, P. W.
Right arrow Articles by Young, L. Y.

 Previous Article  |  Next Article 

Appl Environ Microbiol, May 1998, p. 1650-1656, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification and Analysis of Genes Involved in Anaerobic Toluene Metabolism by Strain T1: Putative Role of a Glycine Free Radical

Peter W. Coschigano,1,* Thomas S. Wehrman,2,dagger and L. Y. Young2

Department of Biological Sciences and Program in Molecular and Cellular Biology, Ohio University, Athens, Ohio 45701-2979,1 and Biotechnology Center for Agriculture and the Environment, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08903-02312

Received 9 December 1997/Accepted 2 March 1998

The denitrifying strain T1 is able to grow with toluene serving as its sole carbon source. Two mutants which have defects in this toluene utilization pathway have been characterized. A clone has been isolated, and subclones which contain tutD and tutE, two genes in the T1 toluene metabolic pathway, have been generated. The tutD gene codes for an 864-amino-acid protein with a calculated molecular mass of 97,600 Da. The tutE gene codes for a 375-amino-acid protein with a calculated molecular mass of 41,300 Da. Two additional small open reading frames have been identified, but their role is not known. The TutE protein has homology to pyruvate formate-lyase activating enzymes. The TutD protein has homology to pyruvate formate-lyase enzymes, including a conserved cysteine residue at the active site and a conserved glycine residue that is activated to a free radical in this enzyme. Site-directed mutagenesis of these two conserved amino acids shows that they are also essential for the function of TutD.

* Corresponding author. Mailing address: Department of Biological Sciences, Ohio University, Athens, OH 45701-2979. Phone: (740) 593-9488. Fax: (740) 593-0300. E-mail: Coschiga{at}ohiou.edu.

dagger Present address: Department of Molecular Pharmacology, Stanford School of Medicine, Stanford University, Stanford, CA 94305-5332.

Appl Environ Microbiol, May 1998, p. 1650-1656, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Carmona, M., Zamarro, M. T., Blazquez, B., Durante-Rodriguez, G., Juarez, J. F., Valderrama, J. A., Barragan, M. J. L., Garcia, J. L., Diaz, E. (2009). Anaerobic Catabolism of Aromatic Compounds: a Genetic and Genomic View. Microbiol. Mol. Biol. Rev. 73: 71-133 [Abstract] [Full Text]  
  • Washer, C. E., Edwards, E. A. (2007). Identification and Expression of Benzylsuccinate Synthase Genes in a Toluene-Degrading Methanogenic Consortium. Appl. Environ. Microbiol. 73: 1367-1369 [Abstract] [Full Text]  
  • Callaghan, A. V., Gieg, L. M., Kropp, K. G., Suflita, J. M., Young, L. Y. (2006). Comparison of Mechanisms of Alkane Metabolism under Sulfate-Reducing Conditions among Two Bacterial Isolates and a Bacterial Consortium.. Appl. Environ. Microbiol. 72: 4274-4282 [Abstract] [Full Text]  
  • Kuhner, S., Wohlbrand, L., Fritz, I., Wruck, W., Hultschig, C., Hufnagel, P., Kube, M., Reinhardt, R., Rabus, R. (2005). Substrate-Dependent Regulation of Anaerobic Degradation Pathways for Toluene and Ethylbenzene in a Denitrifying Bacterium, Strain EbN1. J. Bacteriol. 187: 1493-1503 [Abstract] [Full Text]  
  • Shinoda, Y., Sakai, Y., Uenishi, H., Uchihashi, Y., Hiraishi, A., Yukawa, H., Yurimoto, H., Kato, N. (2004). Aerobic and Anaerobic Toluene Degradation by a Newly Isolated Denitrifying Bacterium, Thauera sp. Strain DNT-1. Appl. Environ. Microbiol. 70: 1385-1392 [Abstract] [Full Text]  
  • Van Hamme, J. D., Singh, A., Ward, O. P. (2003). Recent Advances in Petroleum Microbiology. Microbiol. Mol. Biol. Rev. 67: 503-549 [Abstract] [Full Text]  
  • Raynaud, C., Sarcabal, P., Meynial-Salles, I., Croux, C., Soucaille, P. (2003). Molecular characterization of the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum. Proc. Natl. Acad. Sci. USA 100: 5010-5015 [Abstract] [Full Text]  
  • Kniemeyer, O., Fischer, T., Wilkes, H., Glockner, F. O., Widdel, F. (2003). Anaerobic Degradation of Ethylbenzene by a New Type of Marine Sulfate-Reducing Bacterium. Appl. Environ. Microbiol. 69: 760-768 [Abstract] [Full Text]  
  • Achong, G. R., Rodriguez, A. M., Spormann, A. M. (2001). Benzylsuccinate Synthase of Azoarcus sp. Strain T: Cloning, Sequencing, Transcriptional Organization, and Its Role in Anaerobic Toluene and m-Xylene Mineralization. J. Bacteriol. 183: 6763-6770 [Abstract] [Full Text]  
  • Rabus, R., Wilkes, H., Behrends, A., Armstroff, A., Fischer, T., Pierik, A. J., Widdel, F. (2001). Anaerobic Initial Reaction of n-Alkanes in a Denitrifying Bacterium: Evidence for (1-Methylpentyl)succinate as Initial Product and for Involvement of an Organic Radical in n-Hexane Metabolism. J. Bacteriol. 183: 1707-1715 [Abstract] [Full Text]  
  • Müller, J. A., Galushko, A. S., Kappler, A., Schink, B. (2001). Initiation of Anaerobic Degradation of p-Cresol by Formation of 4-Hydroxybenzylsuccinate in Desulfobacterium cetonicum. J. Bacteriol. 183: 752-757 [Abstract] [Full Text]  
  • Heyen, U., Harder, J. (2000). Geranic Acid Formation, an Initial Reaction of Anaerobic Monoterpene Metabolism in Denitrifying Alcaligenes defragrans. Appl. Environ. Microbiol. 66: 3004-3009 [Abstract] [Full Text]  
  • Coschigano, P. W. (2000). Transcriptional Analysis of the tutE tutFDGH Gene Cluster from Thauera aromatica Strain T1. Appl. Environ. Microbiol. 66: 1147-1151 [Abstract] [Full Text]  
  • Krieger, C. J., Beller, H. R., Reinhard, M., Spormann, A. M. (1999). Initial Reactions in Anaerobic Oxidation of m-Xylene by the Denitrifying Bacterium Azoarcus sp. Strain T. J. Bacteriol. 181: 6403-6410 [Abstract] [Full Text]  
  • Beller, H. R., Spormann, A. M. (1998). Analysis of the Novel Benzylsuccinate Synthase Reaction for Anaerobic Toluene Activation Based on Structural Studies of the Product. J. Bacteriol. 180: 5454-5457 [Abstract] [Full Text]  
  • Krieger, C. J., Roseboom, W., Albracht, S. P. J., Spormann, A. M. (2001). A Stable Organic Free Radical in Anaerobic Benzylsuccinate Synthase of Azoarcus sp. Strain T. J. Biol. Chem. 276: 12924-12927 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»