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Appl Environ Microbiol, May 1998, p. 1657-1662, Vol. 64, No. 5
Department of
Bacteriology,1
Center for the Study of
Nitrogen Fixation,2 and
Department of
Agronomy,3 University of Wisconsin-Madison,
Madison, Wisconsin 53706
Received 6 November 1997/Accepted 19 February 1998
Improved nitrogen-fixing inoculum strains for leguminous crops must
be able to effectively compete with indigenous strains for nodulation,
enhance legume productivity compared to the productivity obtained with
indigenous strains, and maintain stable expression of any added genes
in the absence of selection pressure. We constructed a transposable
element containing the tfx region for expression of
increased nodulation competitiveness and the par locus for plasmid stability. The transposon was inserted into tetA of
pHU52, a broad-host-range plasmid conferring the H2 uptake
phenotype. The resulting plasmid, pHUTFXPAR, conferred the plasmid
stability, trifolitoxin production, and H2 uptake
phenotypes in the broad-host-range organism Sinorhizobium
sp. strain ANU280. The broad applications of a transposon conferring
plasmid stability are discussed.
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
A Transposable Partitioning Locus Used To Stabilize
Plasmid-Borne Hydrogen Oxidation and Trifolitoxin Production Genes
in a Sinorhizobium Strain
*
Corresponding author. Mailing address: Center for the
Study of Nitrogen Fixation, Department of Agronomy, University of
Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706. Phone: (608)
262-9824. Fax: (608) 262-5217. E-mail:
ewtriple{at}facstaff.wisc.edu.
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