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Appl Environ Microbiol, May 1998, p. 1657-1662, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Transposable Partitioning Locus Used To Stabilize Plasmid-Borne Hydrogen Oxidation and Trifolitoxin Production Genes in a Sinorhizobium Strain

Angela D. Kent,1,2 Michelle L. Wojtasiak,2,3 Eduardo A. Robleto,2,3 and Eric W. Triplett1,2,3,*

Department of Bacteriology,1 Center for the Study of Nitrogen Fixation,2 and Department of Agronomy,3 University of Wisconsin-Madison, Madison, Wisconsin 53706

Received 6 November 1997/Accepted 19 February 1998

Improved nitrogen-fixing inoculum strains for leguminous crops must be able to effectively compete with indigenous strains for nodulation, enhance legume productivity compared to the productivity obtained with indigenous strains, and maintain stable expression of any added genes in the absence of selection pressure. We constructed a transposable element containing the tfx region for expression of increased nodulation competitiveness and the par locus for plasmid stability. The transposon was inserted into tetA of pHU52, a broad-host-range plasmid conferring the H2 uptake phenotype. The resulting plasmid, pHUTFXPAR, conferred the plasmid stability, trifolitoxin production, and H2 uptake phenotypes in the broad-host-range organism Sinorhizobium sp. strain ANU280. The broad applications of a transposon conferring plasmid stability are discussed.

* Corresponding author. Mailing address: Center for the Study of Nitrogen Fixation, Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706. Phone: (608) 262-9824. Fax: (608) 262-5217. E-mail: ewtriple{at}facstaff.wisc.edu.

Appl Environ Microbiol, May 1998, p. 1657-1662, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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